Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,957,938	C→T	intergenic (‑699/‑184)	SAUSA300_RS09685 ← / → SAUSA300_RS09690	gamma‑hemolysin subunit A/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,957,938	0	C	T	100.0%	16.0 / NA	7	intergenic (‑699/‑184)	SAUSA300_RS09685/SAUSA300_RS09690	gamma‑hemolysin subunit A/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/7);  total (0/7)

GCTACTACATTCGCATGTACTTCGTTTTACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTCAAAAATCCCCTATGAAAATGTTGATGAGCTACATCTTTAAATCTTATTATACATTGTATAAAATTATATTGCGAGGT  >  NC_007793/1957841‑1958015                                                                                                  |                                                                              gCTACTACATTCGCATGTACTTCGTTTTACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTTaa                                                                             <  1:717983/100‑1 (MQ=255)          ttCGCATGTACTTCGTTTTACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTTAAAAATCCCCt                                                                    <  1:4403163/100‑1 (MQ=255)          ttCGCATGTACTTCGTTTTACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTTAAAAATCCCCt                                                                    <  1:567361/100‑1 (MQ=255)                         ttttACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTTAAAAATCCCCTATGAAAATGTtgatg                                                     <  1:1212740/100‑1 (MQ=255)                         ttttACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAACTTTAAAAATCCCCTATGAAAATGTtgatg                                                     <  1:5550814/100‑1 (MQ=255)                                 tCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTTAAAAATCCCCTATGAAAATGTTGATGAGCTACAt                                             <  1:454165/100‑1 (MQ=255)                                                                            tttattttTCAATACTTAATTTTAAAAATCCCCTATGAAAATGTTGATGAGCTACATCTTTAAATCTTATTATACATTGTATAAAATTATATTGCGAggg  <  1:290451/100‑2 (MQ=255)                                                                                                  |                                                                              GCTACTACATTCGCATGTACTTCGTTTTACTGTCCACGCATGGTCGTTGACTTATTTATATTATAATATAAATATTTTATTTTTCAATACTTAATTTCAAAAATCCCCTATGAAAATGTTGATGAGCTACATCTTTAAATCTTATTATACATTGTATAAAATTATATTGCGAGGT  >  NC_007793/1957841‑1958015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑