Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 1,929,062 G→A intergenic (‑148/‑273) SAUSA300_RS09560 ← / → SAUSA300_RS09565 transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,929,0620GA80.0% 13.0 / ‑1.9 10intergenic (‑148/‑273)SAUSA300_RS09560/SAUSA300_RS09565transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (4/4);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

GAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGC  >  NC_007793/1929020‑1929161
                                          |                                                                                                     
gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCTGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GGTg                                            <  1:5920263/100‑1 (MQ=255)
                      tattcacagatggtATAATAATAGAGTCGCCTATCTCTCGGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TTTGCTAATCATTTTCGTTCAcatcat                     <  1:4047085/92‑1 (MQ=255)
                       ctttGCCGATGATATAACAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GGTGATGCTTATTTTCGTTCAcatcat                     <  1:4670545/99‑1 (MQ=255)
                          cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TTTGCTAATCATTTTCGTTCACATCATGGCa                 >  1:6020183/5‑100 (MQ=255)
                            cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TTTGCTAATCATTTTCGTTCACATCATGGCAcc               >  1:2567913/3‑100 (MQ=255)
                              gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAg             <  1:6029152/100‑1 (MQ=255)
                              gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAg             <  1:940964/100‑1 (MQ=255)
                                tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGtc           <  1:4559012/97‑1 (MQ=255)
                                 ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TTTGCTAATCATTTTCGTTCACATCATAGCACCaagat          >  1:2345257/3‑96 (MQ=255)
                                 ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGtca          >  1:5967744/3‑100 (MQ=255)
                                    ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGtcatct       <  1:2886011/100‑2 (MQ=255)
                                          gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc  <  1:4747805/100‑1 (MQ=255)
                                          |                                                                                                     
GAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGC  >  NC_007793/1929020‑1929161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑