Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,789,112	C→T	G204E (GGA→GAA)	SAUSA300_RS08915 ←	dephospho‑CoA kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,789,112	0	C	T	100.0%	24.7 / NA	10	G204E (GGA→GAA)	SAUSA300_RS08915	dephospho‑CoA kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/8);  total (2/8)

TTTTTATAAAAAATAGTCATTACTTACATACATCAACGCGTACGTAAAGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTCCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAACATG  >  NC_007793/1789026‑1789206                                                                                       |                                                                                               tttttATAAAAAATAGTCATTACTTACATACATCAACGCGTACGTAAAGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTTCGTAATTCGGCtt                                                                                   <  1:1822857/100‑1 (MQ=255) tttttATAAAAAATAGTCATTACTTACATACATCAACGCGTACGTAAAGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTTCGTAATTCGGCtg                                                                                   <  1:2037501/100‑2 (MQ=255)                            atacatCAACGCGTACGTAAAGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTactat                                                        <  1:3477269/100‑5 (MQ=255)                                  atctgcgTACGTAAAGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAAtctctc                                                  <  1:23961/96‑1 (MQ=255)                                                aGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTa                                    >  1:3023597/1‑100 (MQ=255)                                                               ataGTGTAATATTAATCTTCTTCTTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATccc                     <  1:3177473/100‑1 (MQ=255)                                                                    gctcttccgaTCTTCTTCTTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTaaa                <  1:5270822/91‑1 (MQ=255)                                                                            tcttccgatctcCGTCCTTCGGCTTTTCAATATAGCCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGAt        <  1:516478/92‑1 (MQ=255)                                                                            gaTCTTCTTTATCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGAt        <  1:2835726/99‑1 (MQ=255)                                                                               cttcttcttCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAAc     <  1:3943897/100‑1 (MQ=255)                                                                               cttcttcttCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAAc     <  1:4641970/100‑1 (MQ=255)                                                                               cttcttccTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAAc     <  1:1548451/100‑1 (MQ=255)                                                                                ttcttcttcGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAACa    <  1:5616920/100‑1 (MQ=255)                                                                                ccgatcttCGCAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAACa    <  1:1640235/96‑1 (MQ=255)                                                                                 tcttctTCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAACAt   >  1:1049975/1‑100 (MQ=255)                                                                                  cttcttCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAACaag  <  1:1076562/100‑3 (MQ=255)                                                                                       |                                                                                               TTTTTATAAAAAATAGTCATTACTTACATACATCAACGCGTACGTAAAGTAATGACTTATTTATAGTGTAATATTAATCTTCTTCTCCGTAATTCGGCTTTTCAATATAACCTTCTTCTTCTAACAATCTCTCAAGGTTTTGTTTTAATTCAAGTTTATCCCCTAAATTATCGATAACATG  >  NC_007793/1789026‑1789206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑