D37 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	849	A→G	K190K (AAA→AAG)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	849	0	A	G	99.3%	858.2 / ‑4.7	295	K190K (AAA→AAG)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (280/13); total (282/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 300 total aligned reads displayed.

ACTGATGATGATGAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAAC‑AACAGACGAAGAAGAAAAAGCACATTCAA  >  NC_007790/751‑946

 cTGATGACGACGAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCa                                                                                                  >  1:753175/1‑100 (MQ=255)

 cTGATGACGACGAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCa                                                                                                  >  1:199374/1‑100 (MQ=255)

 cTGATGACGACGAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCa                                                                                                  >  1:1511247/1‑100 (MQ=255)

  cagccacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCAt                                                                                                 >  1:4351428/11‑100 (MQ=37)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:2569791/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:2758273/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:3765485/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:2323446/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:165772/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:541658/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa                                                                                                >  1:4512362/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATa                                                                                                >  1:5167519/1‑100 (MQ=255)

   gatgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGACTGCTTAAACAAAAGCATa                                                                                                >  1:5336372/1‑100 (MQ=38)

    gatgacgacgaaaaaaTCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaa                                                                                               >  1:4786680/11‑100 (MQ=37)

    atgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaa                                                                                               >  1:1390735/1‑100 (MQ=255)

     tgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:288663/1‑100 (MQ=255)

     tgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:2597257/1‑100 (MQ=255)

     tgacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:4032636/1‑100 (MQ=255)

     gtacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:1705252/8‑100 (MQ=255)

     ggacggctaaaaaaaTCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:1467127/9‑100 (MQ=37)

     gcacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:3601768/8‑100 (MQ=255)

     caacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa                                                                                              >  1:2184660/8‑100 (MQ=255)

      tacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:4495900/7‑100 (MQ=255)

      tacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:3619575/7‑100 (MQ=255)

      tacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:4751661/7‑100 (MQ=255)

      tacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:1183228/7‑100 (MQ=255)

      gacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAGAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:2137062/7‑100 (MQ=37)

      gacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:2838672/7‑100 (MQ=255)

      gacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:3909788/7‑100 (MQ=255)

      gacgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa                                                                                             >  1:2445748/7‑100 (MQ=255)

       acgacgaAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAAt                                                                                            >  1:2517143/6‑100 (MQ=255)

        cggcgaAAAAAATCAAGAAATTGGAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAAtt                                                                                           >  1:366580/5‑100 (MQ=255)

                     cAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACg                                                                              >  1:3716207/1‑100 (MQ=255)

                     cAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACg                                                                              >  1:5916240/1‑100 (MQ=255)

                     cAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACg                                                                              >  1:4831473/1‑100 (MQ=255)

                       tttaacggTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGAt                                                                            >  1:2668095/8‑100 (MQ=255)

                       ctggcctGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGag                                                                            >  1:3563273/7‑99 (MQ=255)

                       aGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGaa                                                                            >  1:5434333/1‑99 (MQ=255)

                           gttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCg                                                                        >  1:843477/2‑100 (MQ=255)

                           gttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGACg                                                                        >  1:3986839/2‑100 (MQ=255)

                           aTTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCg                                                                        <  1:1006902/100‑1 (MQ=255)

                            ttGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGa                                                                       <  1:5011852/100‑1 (MQ=255)

                             tGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGaa                                                                      >  1:3383167/1‑100 (MQ=255)

                             tGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGaa                                                                      >  1:1195922/1‑100 (MQ=255)

                              gTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAg                                                                     >  1:3021059/1‑100 (MQ=255)

                               caaGTGATTTAGAAAAGGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGa                                                                    >  1:4580941/2‑100 (MQ=37)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:5562047/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:4013067/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:4800810/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:894973/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:4681122/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:4710876/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:2026473/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:1248142/1‑100 (MQ=255)

                                aaGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGAt                                                                   >  1:2629941/1‑100 (MQ=255)

                                 aGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATg                                                                  >  1:5292090/1‑100 (MQ=255)

                                 aGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATg                                                                  >  1:690265/1‑100 (MQ=255)

                                  aactgttAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATgg                                                                 >  1:5892278/6‑100 (MQ=37)

                                    gATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTa                                                               >  1:80505/1‑100 (MQ=255)

                                       ttAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAtt                                                            >  1:5264882/1‑100 (MQ=255)

                                       ccaGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAtt                                                            >  1:1573289/3‑100 (MQ=255)

                                        tAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAttt                                                           >  1:5807459/1‑100 (MQ=255)

                                        tAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAttt                                                           >  1:4902463/1‑100 (MQ=255)

                                           aaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTggg                                                        <  1:2741165/100‑3 (MQ=255)

                                             aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGAtga                                                      >  1:1865488/1‑98 (MQ=255)

                                             aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                                      >  1:2932726/1‑100 (MQ=255)

                                             aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGAGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTa                                                      >  1:1317916/1‑100 (MQ=255)

                                                  tttGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACa                                                 >  1:1423405/1‑100 (MQ=255)

                                                  ttggTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACa                                                 >  1:2392155/4‑100 (MQ=38)

                                                  tgggTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACa                                                 >  1:31719/4‑100 (MQ=38)

                                                   ttGTATCGACAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACaa                                                >  1:2082328/1‑100 (MQ=38)

                                                       aTCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtga                                            >  1:5758275/1‑100 (MQ=255)

                                                          gAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGagatga                                         <  1:5989679/100‑1 (MQ=38)

                                                            aaaCGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAg                                       >  1:5530229/1‑100 (MQ=255)

                                                              aCGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                     >  1:346688/1‑100 (MQ=255)

                                                               cGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATa                                    >  1:5468460/1‑100 (MQ=255)

                                                                atATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaa                                   >  1:3494712/2‑100 (MQ=255)

                                                                  gggTTAAGTTATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaaa                                 >  1:222478/3‑100 (MQ=255)

                                                                          ctATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGat                        >  1:1442539/2‑99 (MQ=255)

                                                                          atATGGTGGATTGCTTAAACAAAAGCATAAAATTTTGAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAGGC‑GACAGat                        >  1:671447/2‑99 (MQ=255)

                                                                           tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAGAC‑GACAGatc                       >  1:3103065/1‑98 (MQ=255)

                                                                           tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GGCAGatc                       >  1:3817255/1‑98 (MQ=255)

                                                                           tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGatc                       >  1:4456548/1‑98 (MQ=255)

                                                                           tATGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑CATAGatc                       >  1:63009/1‑98 (MQ=255)

                                                                           gctggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacggacagat                        >  1:331128/3‑92 (MQ=255)

                                                                            ttggtggATTGCTTAAACAAAAGCATAAAATTTTGAACTTAGACGATGCCGAAGCTGGTAATTTGATTAATACAAGTGATGAAGATAAAACGAACAGatc                       >  1:3945596/2‑98 (MQ=255)

                                                                            ctggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑aacgagatc                      >  1:1094588/2‑94 (MQ=255)

                                                                            ctggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGatcg                      >  1:4380601/2‑97 (MQ=255)

                                                                            ctggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑AACAGatcg                      >  1:3829170/2‑97 (MQ=255)

                                                                            aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacccagatcgg                       >  1:727524/1‑91 (MQ=255)

                                                                            aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑gactagatc                      >  1:1747073/1‑94 (MQ=255)

                                                                            aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAGC‑GACAGatcg                      >  1:1800831/1‑97 (MQ=255)

                                                                            aTGGTGGATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GCCAGatcg                      >  1:4535430/1‑97 (MQ=255)

                                                                              ggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAT‑GACAGatcgga                    >  1:5364243/1‑95 (MQ=255)

                                                                              ggtggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGatcgga                    >  1:2698508/1‑95 (MQ=255)

                                                                              aggggATTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GCCAGatcgga                    >  1:507667/4‑95 (MQ=255)

                                                                                  taTTGCTTAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAATAC‑GACAGatcggaagag                >  1:1371052/2‑91 (MQ=255)

                                                                                       tatAAACAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGc          >  1:4213034/3‑100 (MQ=255)

                                                                                          aaaCAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGcaca       >  1:5726049/1‑100 (MQ=255)

                                                                                              aaaagCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAA‑AAAAAGCACATTCa   >  1:4128301/1‑100 (MQ=255)

ACTGATGATGATGAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAAC‑AACAGACGAAGAAGAAAAAGCACATTCAA  >  NC_007790/751‑946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`