Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,630,757	T→A	intergenic (‑170/+185)	SAUSA300_RS08055 ← / ← SAUSA300_RS08060	acetyl‑CoA carboxylase biotin carboxyl carrier protein subunit/integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,630,757	0	T	A	100.0%	15.6 / NA	7	intergenic (‑170/+185)	SAUSA300_RS08055/SAUSA300_RS08060	acetyl‑CoA carboxylase biotin carboxyl carrier protein subunit/integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/7);  total (0/7)

TTTTAAATGGCAGTGTGTCAATAGAAAAATGAGAATTTCTTAATTTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAG  >  NC_007793/1630714‑1630837                                            |                                                                                 aattAAATGGCAGTGTGTCAATAGAAAAATGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACt                          <  1:5458844/98‑1 (MQ=255)                       aGAAAAATGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACcac    <  1:145523/100‑1 (MQ=255)                       aGAAAAATGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACcac    <  1:2840574/100‑1 (MQ=255)                       aGAAAAATGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACcac    <  1:3034250/100‑1 (MQ=255)                       aGAAAAATGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACcac    <  1:5255457/100‑1 (MQ=255)                       aGAAAAATGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACcaa    <  1:5112628/100‑2 (MQ=255)                         aaaaaTGAGAATTTCTTAAATTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAg  <  1:1058864/100‑1 (MQ=255)                                            |                                                                                 TTTTAAATGGCAGTGTGTCAATAGAAAAATGAGAATTTCTTAATTTTTTTTAATAAATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAG  >  NC_007793/1630714‑1630837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑