Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,295,497	A→G	A207A (GCA→GCG)	SAUSA300_RS06365 →	competence protein CinA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,295,497	0	A	G	100.0%	107.7 / NA	38	A207A (GCA→GCG)	SAUSA300_RS06365	competence protein CinA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/1);  total (37/1)

TCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAC  >  NC_007793/1295405‑1295588                                                                                             |                                                                                            tCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTgg                                                                                      >  1:3046063/1‑100 (MQ=255)    gAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCgg                                                                                   >  1:1887194/1‑100 (MQ=255)         aTTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGt                                                                              >  1:5271489/1‑100 (MQ=255)           tAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCa                                                                            >  1:3829331/1‑100 (MQ=255)                 ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                      >  1:1063/1‑100 (MQ=255)                  tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:429197/1‑100 (MQ=255)                  tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:2008801/1‑100 (MQ=255)                  tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:2639876/1‑100 (MQ=255)                  agCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:2703433/2‑100 (MQ=255)                   gCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtat                                                                    >  1:829963/1‑100 (MQ=255)                   gCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtat                                                                    >  1:799851/1‑100 (MQ=255)                    cGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtata                                                                   >  1:814550/1‑100 (MQ=255)                        caTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATAtt                                                               >  1:4591037/2‑100 (MQ=255)                         aTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTa                                                              >  1:1118928/1‑100 (MQ=255)                         aTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTa                                                              >  1:4002163/1‑100 (MQ=255)                           aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                            >  1:3914996/1‑100 (MQ=255)                            tagttctCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGAt                                                           >  1:4771405/7‑100 (MQ=255)                                     aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                  >  1:3531999/1‑100 (MQ=255)                                     aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                  >  1:3923544/1‑100 (MQ=255)                                     aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGAAAGTCATGAGGTAAATATTAGATTAACTGCaa                                                  >  1:4814237/1‑100 (MQ=255)                                        ctAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATg                                               >  1:4079775/2‑100 (MQ=255)                                                      aaTAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGaa                                 >  1:2596864/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:3987990/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:1025569/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:1092898/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:1926154/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCGTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAGCAAGCACaa                        >  1:1822654/1‑100 (MQ=255)                                                                aTCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAAt                       >  1:2765815/1‑100 (MQ=255)                                                                        ccAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGAtt               >  1:2854887/2‑100 (MQ=255)                                                                                gcccTACTGTTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGt       >  1:1459612/3‑100 (MQ=255)                                                                                 tCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGtt      >  1:3810161/1‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     <  1:3738922/100‑1 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:5939192/1‑100 (MQ=255)                                                                                    tACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAATAACAAGCACAATCATTGATTCAACCTGTTaaa   >  1:268902/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:1872849/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:5425455/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:5440413/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:2642775/1‑100 (MQ=255)                                                                                             |                                                                                            TCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAC  >  NC_007793/1295405‑1295588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑