| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,018,825 | T→A | noncoding (24/89 nt) | SAUSA300_RS04975 ← | tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,018,825 | 0 | T | A | 88.9% | 16.5 / ‑2.7 | 10 | noncoding (24/89 nt) | SAUSA300_RS04975 | tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (2/6); total (3/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01 | |||||||||||
AATGGTATATACCATTTTAGCAAGTAACGGAAACGGAGGGATTCGAACCCTCGCGCCGCTTTCGCGACCTACACCCTTAGCAGGGGCGCCTCTTCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTGA > NC_007793/1018733‑1018914 | aaTCGTATCTCCCATTTTAGCAAGTAACGGAAACGGAGGGATTCGAACCCTCGCGCCGCTTTCGCGCCCCGCACCCTTAGCAGGGGCGCCTCATCAGCCa < 1:3878630/100‑1 (MQ=255) acacCCTTAGCAGGGGCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAATAGCCGATAg > 1:1824280/1‑100 (MQ=255) acacCCTTAGCAGGGGCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAg > 1:862480/1‑100 (MQ=255) tAGCAGGGGCGCCTCTTCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTAcc > 1:4383761/1‑100 (MQ=255) cAGGGGCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACt < 1:131529/100‑1 (MQ=255) aGGGGCGCCTCCTCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTg < 1:1635784/100‑1 (MQ=255) aGGGGCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTg < 1:1280272/100‑1 (MQ=255) aGGGGCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTg < 1:3842353/100‑1 (MQ=255) aGGGGCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTg < 1:4167419/100‑1 (MQ=255) ggggCGCCTCATCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTgg < 1:3435902/100‑2 (MQ=255) | AATGGTATATACCATTTTAGCAAGTAACGGAAACGGAGGGATTCGAACCCTCGCGCCGCTTTCGCGACCTACACCCTTAGCAGGGGCGCCTCTTCAGCCAACTTGAGTACGTTTCCAATGGCTCCACAGGTAGGACTCGAACCTACGACCGATCGGTTAACAGCCGATAGCTCTACCACTGA > NC_007793/1018733‑1018914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |