Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	893,036	G→T	intergenic (+93/‑336)	SAUSA300_RS04385 → / → SAUSA300_RS04390	hypothetical protein/CAAX amino protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	893,036	0	G	T	100.0%	36.4 / NA	13	intergenic (+93/‑336)	SAUSA300_RS04385/SAUSA300_RS04390	hypothetical protein/CAAX amino protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/1);  total (12/1)

GGGACGGAGATGATGAGGAATTAGATAAGGCAGTGCAAGATGTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAATTAATA  >  NC_007793/892995‑893128                                          |                                                                                             atgccGGAGATGATGAGGAATTAGATAAGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTg                                    >  1:3484229/5‑100 (MQ=255)          atgatgAGGAATTAGATAAGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATg                           <  1:5515389/100‑1 (MQ=255)                         ctAAGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTta            >  1:3164208/2‑100 (MQ=255)                          tAAGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTtat           >  1:1713312/1‑100 (MQ=255)                           aaGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGCTTGAAGAAAATGAAGCTATGCTATTtata          >  1:3691717/1‑100 (MQ=255)                           aaGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTtata          >  1:3680113/1‑100 (MQ=255)                           aaGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTtata          >  1:5224503/1‑100 (MQ=255)                           aaGGCAGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTtata          >  1:5478635/1‑100 (MQ=255)                               taGTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAAtt      >  1:3114334/2‑100 (MQ=255)                                 gTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAATTaa    >  1:1199010/1‑100 (MQ=255)                                 gTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAATTaa    >  1:2169222/1‑100 (MQ=255)                                 gTGCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAATTaa    >  1:723759/1‑100 (MQ=255)                                   gCAAGATTTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAATTAATa  >  1:1155592/1‑100 (MQ=255)                                          |                                                                                             GGGACGGAGATGATGAGGAATTAGATAAGGCAGTGCAAGATGTATCTAACGCTAACCATAATCATACTGTGATTGTGGATGATATACCGTTAGAGGATTGAAGAAAATGAAGCTATGCTATTTATAAATTAATA  >  NC_007793/892995‑893128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑