Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007790 759 T→C D160D (GAT→GAC)  SAUSA300_RS14705 → replication protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077907590TC100.0% 508.4 / NA 172D160D (GAT→GAC) SAUSA300_RS14705replication protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (153/19);  total (153/19)

Only 100 of 228 total aligned reads displayed.

TCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATT  >  NC_007790/700‑858
                                                           |                                                                                                    
cacccactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                                                             >  1:520800/11‑100 (MQ=255)
 taacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg                                                            >  1:5421800/10‑100 (MQ=255)
 atacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg                                                            >  1:4765385/10‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCGTCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt                                                           >  1:4233513/9‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg                                                            >  1:3129256/9‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt                                                           >  1:5851325/9‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt                                                           >  1:5120625/9‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt                                                           >  1:1519846/9‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt                                                           >  1:4006692/9‑100 (MQ=255)
  aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt                                                           >  1:291100/9‑100 (MQ=255)
   tcactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAggat                                                          >  1:3609182/8‑98 (MQ=255)
   acactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt                                                          >  1:823842/8‑100 (MQ=255)
   acactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt                                                          >  1:1226597/8‑100 (MQ=255)
    cactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACA‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGttt                                                         >  1:4067264/7‑100 (MQ=255)
      ctcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGt                                                       >  1:5412572/5‑100 (MQ=255)
        cgaaGAGACATCAAAATATTCCGTTAAATCCTCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt                                                     >  1:933993/3‑100 (MQ=255)
        cgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt                                                     >  1:4493320/3‑100 (MQ=255)
         ccagagACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATc                                                    >  1:1633373/3‑100 (MQ=255)
            ctgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaa                                                 >  1:5934962/3‑100 (MQ=255)
             tgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa                                                >  1:5399461/2‑100 (MQ=255)
             ggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaa                                                 >  1:4404528/2‑100 (MQ=255)
             ggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa                                                >  1:5905747/2‑100 (MQ=255)
             cgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa                                                >  1:569423/2‑100 (MQ=255)
             agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCaaaa                                                >  1:221295/1‑100 (MQ=255)
             agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa                                                >  1:2942558/1‑100 (MQ=255)
             agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa                                                >  1:3894046/1‑100 (MQ=255)
              tacatCAAAATATTCCGTTAAATCATCTGA‑CTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAAc                                              >  1:5033417/5‑100 (MQ=255)
              gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa                                               >  1:229837/1‑100 (MQ=255)
              gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa                                               >  1:2410384/1‑100 (MQ=255)
              gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa                                               >  1:5077404/1‑100 (MQ=255)
              gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa                                               >  1:5256506/1‑100 (MQ=255)
              gaCATCAAAATATTCCGTTAAATCATCTGACCTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGATTGTATCGaaaa                                               >  1:3211523/1‑100 (MQ=255)
                catCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACg                                             >  1:1804114/3‑100 (MQ=255)
                   cccggcatTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTAt                                          >  1:5485925/7‑100 (MQ=255)
                   cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTa                                           >  1:2776859/1‑100 (MQ=255)
                   cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTAt                                          >  1:4515747/1‑100 (MQ=255)
                   cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTAt                                          >  1:1053008/1‑100 (MQ=255)
                   cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTAt                                          >  1:5592680/1‑100 (MQ=255)
                       atatTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTa                                      <  1:2243138/100‑1 (MQ=38)
                        caggccgTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTaa                                     >  1:1430897/7‑100 (MQ=37)
                          ctccgTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGt                                   >  1:5052367/5‑100 (MQ=37)
                            ccgTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTa                                 >  1:829227/3‑100 (MQ=37)
                                tAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGGAATTGTAAGTGATTTAGAAGAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                             <  1:554601/100‑1 (MQ=37)
                                tAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                             >  1:342076/1‑100 (MQ=38)
                                tAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                             <  1:3669135/100‑1 (MQ=255)
                                 ttccgatcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtg                            <  1:618255/95‑1 (MQ=38)
                                  aaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgc                           <  1:1968946/100‑2 (MQ=255)
                                   atcatcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGa                          <  1:1534908/100‑1 (MQ=255)
                                      atcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGAttt                       <  1:731816/100‑2 (MQ=255)
                                       tcTGACTTTTAAACTGATGACGACG‑AAAAGAATCAAGAATTTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGGTAAGTTATGGTAGAtcgg                      >  1:4974028/1‑97 (MQ=25)
                                          gactttttAACTGGTGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTa                   >  1:5282016/4‑100 (MQ=37)
                                          gactttttAACTGATGACGACG‑AAAAAAATCAGGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTa                   >  1:248549/4‑100 (MQ=37)
                                          gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTa                   >  1:176587/4‑100 (MQ=38)
                                          gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTa                   >  1:1398590/4‑100 (MQ=38)
                                          gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTa                   >  1:2972745/4‑100 (MQ=255)
                                          gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTa                   >  1:5509150/4‑100 (MQ=38)
                                          gactttttAACTGATGACAACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTACGGTAGAtcggaag                   >  1:4879502/4‑94 (MQ=25)
                                              ccttAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACa               >  1:3701389/3‑100 (MQ=255)
                                                ttAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaa             >  1:4125062/1‑100 (MQ=255)
                                                 tAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaa            >  1:2184370/1‑100 (MQ=255)
                                                 tAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaa            >  1:4868220/1‑100 (MQ=255)
                                                 tAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaa            >  1:168617/1‑100 (MQ=255)
                                                  cccTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:2283359/3‑99 (MQ=255)
                                                  caCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:4569336/2‑99 (MQ=255)
                                                  aaCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:4715780/1‑99 (MQ=255)
                                                  aaCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:1120924/1‑99 (MQ=255)
                                                  aaCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:1155420/1‑99 (MQ=255)
                                                  aaCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:5453294/1‑99 (MQ=255)
                                                  aaCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:3732410/1‑99 (MQ=255)
                                                  aaCTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACATATGTTAAGTTATGGTGGATTGCTTAAACaaaag           >  1:5063941/1‑99 (MQ=255)
                                                   ccTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaagc          >  1:4799884/2‑98 (MQ=255)
                                                   ccTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaagc          >  1:4830182/2‑98 (MQ=255)
                                                   ccTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACaaaagc          >  1:4890118/2‑98 (MQ=255)
                                                    cTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCa         >  1:199374/1‑100 (MQ=255)
                                                    cTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCa         >  1:753175/1‑100 (MQ=255)
                                                    cTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCa         >  1:2583597/1‑100 (MQ=255)
                                                     tgatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAGCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCAt        >  1:4662593/1‑100 (MQ=38)
                                                     gcctgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCAt        >  1:3331624/4‑100 (MQ=37)
                                                      tatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:5324542/2‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:3650993/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:4142015/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:3922015/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:3382584/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:165772/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:284692/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:3765485/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:274254/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:2323446/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:67965/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATa       >  1:2569791/1‑100 (MQ=255)
                                                      gatgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATa       >  1:5167519/1‑100 (MQ=255)
                                                       atgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaa      >  1:1390735/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:4511643/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:5616636/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:1450760/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:2597257/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:288663/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:5357868/1‑100 (MQ=255)
                                                        tgacgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGGATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaa     >  1:4520569/1‑100 (MQ=37)
                                                         cgtgacg‑aAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAGCATaaaa    >  1:357552/3‑100 (MQ=255)
                                                           |                                                                                                    
TCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATT  >  NC_007790/700‑858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑