Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	425,833	A→T	E106D (GAA→GAT)	SAUSA300_RS02000 →	phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	425,833	0	A	T	91.7%	27.0 / ‑3.8	12	E106D (GAA→GAT)	SAUSA300_RS02000	phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (11/0);  total (12/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

ACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGAAAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGTTTTTCACAAAAAGCGCCTGAAGGAGAAAGTTATGAA  >  NC_007793/425753‑425911                                                                                 |                                                                               aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:2767802/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:2866089/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:287708/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:3220254/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:3447769/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:804102/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGAAAAATATTTCAATGATCCaa                                                             >  1:3225772/1‑100 (MQ=255) aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGGGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                                                             >  1:4832736/1‑100 (MQ=255)         aTTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTaaa                                                     >  1:1185549/1‑100 (MQ=255)         aTTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTaaa                                                     >  1:4357508/1‑100 (MQ=255)                           tGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGtttt                                   >  1:804326/1‑100 (MQ=255)                                                            agacccGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGTTTTTCACAAAAAGCGCCTGAAGGAGAAAGTTATGaa  >  1:5054930/6‑100 (MQ=255)                                                                                 |                                                                               ACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGAAAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGTTTTTCACAAAAAGCGCCTGAAGGAGAAAGTTATGAA  >  NC_007793/425753‑425911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑