Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,057	C→T	D258N (GAC→AAC)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,057	0	C	T	100.0%	55.0 / NA	20	D258N (GAC→AAC)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/15);  total (5/15)

AACTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTG  >  NC_007793/375968‑376143                                                                                          |                                                                                       aaCTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTcc                                                                              >  1:5748305/1‑100 (MQ=255)                             gtACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGACCATGACCCAATTGATTAATTGGTTGAACt                                                  >  1:4967153/1‑100 (MQ=255)                                         ttttGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGtat                                      <  1:186119/100‑1 (MQ=255)                                                      gTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACt                         <  1:5247145/100‑1 (MQ=255)                                                       tCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTg                        <  1:4743616/100‑1 (MQ=255)                                                         gACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTAAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATAGTATATCCCAAATCACTGcc                      <  1:1678663/100‑1 (MQ=255)                                                         gACCCCAGCCAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGcc                      <  1:2157408/100‑1 (MQ=255)                                                          aCCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGcca                     <  1:1941183/100‑2 (MQ=255)                                                          aCCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCct                     >  1:5293260/1‑100 (MQ=255)                                                           ccccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctc                    >  1:1148910/1‑100 (MQ=255)                                                                          ccTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCg     >  1:4402476/2‑100 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCgc    <  1:3608307/100‑2 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt    <  1:3848824/100‑1 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt    <  1:5427650/100‑1 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt    <  1:4941367/100‑1 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt    <  1:3101966/100‑1 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt    <  1:3083913/100‑1 (MQ=255)                                                                           cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt    <  1:1198397/100‑1 (MQ=255)                                                                            tAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtg   <  1:1911245/100‑2 (MQ=255)                                                                             aaGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtcg  <  1:434012/100‑3 (MQ=255)                                                                                          |                                                                                       AACTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTG  >  NC_007793/375968‑376143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑