Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	331,125	G→A	R76R (AGG→AGA)	SAUSA300_RS01495 →	type VII secretion protein EsaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	331,125	0	G	A	100.0%	13.3 / NA	6	R76R (AGG→AGA)	SAUSA300_RS01495	type VII secretion protein EsaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/2);  total (4/2)

TAATAAAATACATATCGCAATTGTTAACGAGGATCAACCAACGACATATAACGGTAAAAAAGTTGAGCTGGGTCAAGCATTTATTAAAAGGTTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTCTGGTTTGAAAAATGGTGGATACCAAGTCATGATTGTTA  >  NC_007793/331035‑331219                                                                                           |                                                                                               taataaAATACATATCGCAATTGTTAACGAGGATCAACCAACGACATATAACGGTAAAAAAGTTGAGCTGGGTCAAGCATTTATTAAAAGATTAGCatag                                                                                       <  1:44472/100‑4 (MQ=255)                                                cttaCGGTAAAAAAGTTGAGCTGGGTCAAGCATTTATTAAAAGATTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTc                                        >  1:352538/4‑100 (MQ=255)                                                                   tcTGGGTCAAGCATTTATTAAAAGATTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTCTGGTTTGAAAAATGGTGGa                     >  1:1388080/2‑100 (MQ=255)                                                                      gggTCAAGCATTTATTAAAAGATTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTCTGGTTTGAAAAATGGTGGATAc                  >  1:3353347/1‑100 (MQ=255)                                                                      gggTCAAGCATTTATTAAAAGATTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTCTGGTTTGAAAAATGGTGGATAc                  >  1:773479/1‑100 (MQ=255)                                                                                      aaaaGATTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTCTGGTTTGAAAAATGGTGGATACCAAGTCATGATTGttn  <  1:4232941/100‑2 (MQ=255)                                                                                           |                                                                                               TAATAAAATACATATCGCAATTGTTAACGAGGATCAACCAACGACATATAACGGTAAAAAAGTTGAGCTGGGTCAAGCATTTATTAAAAGGTTAGCAAATGAGAAAAACTATAAATTTGAAACAGTAACAAGAAACGTTGCTGAGTCTGGTTTGAAAAATGGTGGATACCAAGTCATGATTGTTA  >  NC_007793/331035‑331219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑