Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	240,358	T→A	N358K (AAT→AAA)	SAUSA300_RS01070 →	peptide ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	240,358	0	T	A	100.0%	48.1 / NA	17	N358K (AAT→AAA)	SAUSA300_RS01070	peptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/2);  total (15/2)

AGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAC  >  NC_007793/240276‑240426                                                                                   |                                                                     aGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATgg                                                     >  1:3575171/1‑100 (MQ=255)            cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                          >  1:99466/1‑100 (MQ=255)            cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                          >  1:5197862/1‑100 (MQ=255)            cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                          >  1:3413149/1‑100 (MQ=255)                 gCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAaataat                                     >  1:2192594/1‑100 (MQ=255)                   gTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAaataaatc                                   <  1:1122521/100‑4 (MQ=255)                             cagcAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTAtt                         >  1:59629/4‑100 (MQ=255)                             aGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTAtt                         >  1:4012167/1‑100 (MQ=255)                                cAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAACAATAAAGatcggaagagc                      >  1:4217967/1‑90 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATgacgac  >  1:3448105/1‑100 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTAGGAc  >  1:1306944/1‑100 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACggc  <  1:1209687/100‑3 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGaa  >  1:4417099/1‑99 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc  >  1:2635116/1‑100 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc  >  1:5300178/1‑100 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc  >  1:5352114/1‑100 (MQ=255)                                                    aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc  >  1:970571/1‑100 (MQ=255)                                                                                   |                                                                     AGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAC  >  NC_007793/240276‑240426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑