Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	717	C→A	T146T (ACC→ACA)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	717	0	C	A	100.0%	223.5 / NA	72	T146T (ACC→ACA)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (70/2);  total (70/2)

TCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAA  >  NC_007790/700‑813                  |                                                                                                  gggccactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                >  1:5068987/11‑100 (MQ=255) ctaacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                >  1:5503773/11‑100 (MQ=255) cacccactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                >  1:520800/11‑100 (MQ=255) ataacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                >  1:2923452/11‑100 (MQ=255) ataacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                >  1:2630431/11‑100 (MQ=255) ataacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAg                >  1:5889292/11‑100 (MQ=255)  taacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg               >  1:5421800/10‑100 (MQ=255)  atacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg               >  1:4765385/10‑100 (MQ=255)   cacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:5829283/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCGTCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:4233513/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg               >  1:3129256/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgga              >  1:956411/9‑99 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:5812252/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:5528067/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:5851325/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:5120625/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:4006692/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:3585548/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:4709477/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:291100/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:1519846/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:2166547/9‑100 (MQ=255)   aacactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGt              >  1:2581656/9‑100 (MQ=255)    tcactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAggat             >  1:3609182/8‑98 (MQ=255)    ccactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt             >  1:3660037/8‑100 (MQ=255)    ccactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt             >  1:4888556/8‑100 (MQ=255)    acactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt             >  1:1226597/8‑100 (MQ=255)    acactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt             >  1:823842/8‑100 (MQ=255)    acactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt             <  1:2304890/93‑1 (MQ=255)    acactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTCAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGtt             >  1:5588745/8‑100 (MQ=255)     ccctcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGttt            >  1:517141/7‑100 (MQ=255)     cactcgaaGAGACATCAGAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGttt            <  1:1525180/94‑1 (MQ=255)     cactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGttt            >  1:2783872/7‑100 (MQ=255)     cactcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACA‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGttt            >  1:4067264/7‑100 (MQ=255)      cctcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACCGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTg           >  1:5385068/6‑100 (MQ=255)       ctcgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGt          >  1:5412572/5‑100 (MQ=255)         cgaaGAGACATCAAAATATTCCGTTAAATCCTCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt        >  1:933993/3‑100 (MQ=255)         cgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt        >  1:4493320/3‑100 (MQ=255)         cgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt        >  1:4962983/3‑100 (MQ=255)         cgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt        >  1:3707978/3‑100 (MQ=255)         cgaaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTAt        >  1:1823714/3‑100 (MQ=255)          ccagagACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATc       >  1:1633373/3‑100 (MQ=255)            ctagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGa     >  1:3788997/3‑100 (MQ=255)            cctgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGa     >  1:3013273/4‑100 (MQ=255)            agagACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGa     >  1:114282/1‑100 (MQ=255)            acagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGa     >  1:5412526/3‑100 (MQ=255)             gagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaa    >  1:5352579/1‑100 (MQ=255)             ctgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaa    >  1:1781697/3‑100 (MQ=255)             ctgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaa    >  1:5934962/3‑100 (MQ=255)              tgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:5399461/2‑100 (MQ=255)              ggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaa    >  1:4404528/2‑100 (MQ=255)              ggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:5905747/2‑100 (MQ=255)              ggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:4594466/2‑100 (MQ=255)              cgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:569423/2‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCaaaa   >  1:221295/1‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:2942558/1‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:849209/1‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:5609060/1‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:1646717/1‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:5301329/1‑100 (MQ=255)              agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:3894046/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaa   >  1:1985250/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTTAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:662995/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:4842300/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:2410384/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:758783/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:4805610/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:229837/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:5077404/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:3735876/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGaaaa  >  1:5256506/1‑100 (MQ=255)               gaCATCAAAATATTCCGTTAAATCATCTGACCTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGATTGTATCGaaaa  >  1:3211523/1‑100 (MQ=255)                  |                                                                                                  TCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAA  >  NC_007790/700‑813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑