Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,111,0400AT65.0% 523.0 / inf 498E702D (GAA→GAT) SAUSA300_RS05455pyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base A (161/10);  new base T (318/0);  total (488/10)
Fisher's exact test for biased strand distribution p-value = 2.29e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-03

Only 100 of 512 total aligned reads displayed.

CTCAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGAT  >  NC_007793/1110941‑1111139
                                                                                                   |                                                                                                   
  cAGAGGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:1726964/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATGCAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:2929335/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGTGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:1148020/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:5566067/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:1240439/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:4686025/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGGGTTAGATCg                                                                                                   >  1:4440858/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:3105508/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:5440527/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:10109/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:3646857/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:5325528/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:242742/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:5114378/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:5909770/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:3260774/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:1322331/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:1280785/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTCAGATCg                                                                                                   >  1:4972323/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTCAGATCg                                                                                                   >  1:3981954/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGCTAGATCg                                                                                                   >  1:3449813/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAATAGTTAGATCg                                                                                                   >  1:2511858/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGTGTTAGATCg                                                                                                   >  1:3538617/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5548430/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:903589/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5666289/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:3321640/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5683656/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:1489066/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:780546/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:3715839/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:1899674/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:1932557/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAGCg                                                                                                   >  1:5618797/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAAca                                                                                                   >  1:625521/1‑99 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:4292271/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:413100/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:5079471/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:486311/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:3002175/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1624403/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1847512/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:2178222/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:2363125/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:5857122/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:5740189/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1367926/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:2814261/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTCAGATCg                                                                                                   >  1:1741968/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTCAGATCg                                                                                                   >  1:859716/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTAAGATCg                                                                                                   >  1:4241500/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTAAGATCg                                                                                                   >  1:1074277/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGGTAGATCg                                                                                                   >  1:5282487/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:2597702/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:3599206/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:232768/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:440711/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:3044253/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGACTTAGATCg                                                                                                   >  1:5482841/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATTAGATCg                                                                                                   >  1:4571069/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATCAGATCg                                                                                                   >  1:1577623/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAG‑GTTAGATCgg                                                                                                  >  1:1979516/1‑99 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAG‑GTTAGATCgg                                                                                                  >  1:2106822/1‑99 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAATGTTAGATCg                                                                                                   >  1:3474853/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAGTTAGATCg                                                                                                   >  1:389370/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAGTTAGATCg                                                                                                   >  1:1520035/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAGTTAGATCg                                                                                                   >  1:2230738/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAGTTAGATCg                                                                                                   >  1:3210708/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAGTCAGATCg                                                                                                   >  1:1416441/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGAATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:2794473/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTCTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATTAGATCg                                                                                                   >  1:3832356/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTAACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:3672293/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGCGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTCAGATCg                                                                                                   >  1:4029338/1‑100 (MQ=255)
     caGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaa                                                                                                >  1:832061/2‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCggaa                                                                                                >  1:3771435/1‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaa                                                                                                >  1:4655900/1‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaa                                                                                                >  1:2701665/1‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaa                                                                                                >  1:4376292/1‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGa                                                                                                <  1:4777502/100‑1 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGa                                                                                                >  1:2672637/1‑100 (MQ=255)
      aGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGaa                                                                                               >  1:5161898/1‑100 (MQ=255)
       ggTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaaga                                                                                              >  1:6969/1‑95 (MQ=255)
       cctACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggagag                                                                                              >  1:5792633/3‑95 (MQ=255)
        gTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATTAGATCggaagag                                                                                             >  1:4500819/1‑94 (MQ=255)
        ctACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTTAAACTAGCTAAAGAGTTAGAAAGTGAAga                                                                                             >  1:2539041/2‑99 (MQ=255)
          tcTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaagagca                                                                                           >  1:4154244/2‑92 (MQ=255)
          aCTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGtt                                                                                           >  1:3859610/1‑100 (MQ=255)
                    ataCAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATAtttt                                                                                 >  1:3925833/1‑100 (MQ=255)
                                                    aaCATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAAc                                                 >  1:2748445/1‑100 (MQ=255)
                                                                       cctatGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAatt                              >  1:5016969/3‑100 (MQ=255)
                                                                              cAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGt                       >  1:645678/1‑100 (MQ=255)
                                                                               aaaCTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTg                      <  1:1211144/100‑1 (MQ=255)
                                                                                    aGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGGCGCTTACGAATTGATTGGTGAGTTa                 >  1:1660831/1‑100 (MQ=255)
                                                                                    aGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTa                 >  1:599431/1‑100 (MQ=255)
                                                                                          agccttAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAACCGCTTACGAATTGATTGGTGAGTTAAAATCa           >  1:2191757/5‑100 (MQ=255)
                                                                                               tAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGcgta      >  1:521763/1‑97 (MQ=255)
                                                                                               tAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGt      >  1:953680/1‑100 (MQ=255)
                                                                                               tAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTAAGAATTGATTGGTGAGTTAAAATCAGCtta      >  1:2869246/1‑98 (MQ=255)
                                                                                               ctccaCGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGt      >  1:1787391/5‑100 (MQ=255)
                                                                                                  caCGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGa   >  1:34116/2‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTCAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGAT  >  NC_007793/1110941‑1111139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑