Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	256,666	+CC	intergenic (+306/+53)	SAUSA300_RS01135 → / ← SAUSA300_RS01140	antiporter/DNA‑binding response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	256,664	1	.	C	100.0%	26.7 / NA	12	intergenic (+304/+55)	SAUSA300_RS01135/SAUSA300_RS01140	antiporter/DNA‑binding response regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/12);  total (0/12)
*	NC_007793	256,664	2	.	C	100.0%	26.0 / NA	12	intergenic (+304/+55)	SAUSA300_RS01135/SAUSA300_RS01140	antiporter/DNA‑binding response regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/12);  total (0/12)

TGTAGGGAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGT‑‑CCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTAATCTATTTTGCTTGCTTACAATAATCACTTGGCG  >  NC_007793/256591‑256750                                                                           ||                                                                                       tGTAGGGAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATgg                                                                <  1:189957/100‑1 (MQ=255) tGTAGGGAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATgg                                                                <  1:1980639/100‑1 (MQ=255) tGTAGGGAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATgg                                                                <  1:5575919/100‑1 (MQ=255) tGTAGGGAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATgg                                                                <  1:910847/100‑1 (MQ=255)     gggAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAggt                                                            <  1:5827890/100‑2 (MQ=255)     gggAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAGgc                                                            <  1:2202888/100‑1 (MQ=255)       gAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAGgcgt                                                          <  1:2054037/100‑2 (MQ=255)                              ctctAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTag                                   <  1:1530990/100‑2 (MQ=255)                                                 agaaaAGTGAAAACTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTAATCTATTTTGCTTGCTTACa                <  1:2839988/100‑1 (MQ=255)                                                          aaatCTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTAATCTATTTTGCTTGCTTACAATAATCACt       <  1:2117054/100‑1 (MQ=255)                                                              cTTCTCTATCAGTCCCCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTAATCTATTTTGCTTGCTTACAATAATCACTTGGc   <  1:1430399/100‑1 (MQ=255)                                                               ttCTCTCTCAGTCCCCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTAATCTATTTTGCTTGCTTACAATAATCACTTGGCg  <  1:3989425/100‑1 (MQ=255)                                                                           ||                                                                                       TGTAGGGAGTATTGTTTTATATTTAAACTCTCTAAAAAGCGGACTGAAAGAAAAGTGAAAACTTCTCTATCAGT‑‑CCGCTTTTTCATAGAACAAAATGGAGGCGCCATAATCATTAGTTATGTGCTAATCTATTTTGCTTGCTTACAATAATCACTTGGCG  >  NC_007793/256591‑256750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑