Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	277,817	G→A	S179S (TCG→TCA)	SAUSA300_RS01220 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	277,817	0	G	A	100.0%	261.9 / NA	91	S179S (TCG→TCA)	SAUSA300_RS01220	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (80/11);  total (80/11)

TGGATTATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCGGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTACTA  >  NC_007793/277730‑277913                                                                                        |                                                                                                 tGGATTATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGttcttc                                                                                      >  1:4646931/1‑100 (MQ=255)  ggATTATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTtcttct                                                                                     >  1:314650/1‑100 (MQ=255)  ggATTATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTtcttct                                                                                     >  1:4973203/1‑100 (MQ=255)      tATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTa                                                                                 >  1:3325148/1‑100 (MQ=255)      tATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTa                                                                                 >  1:844395/1‑100 (MQ=255)      tATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTa                                                                                 >  1:3589311/1‑100 (MQ=255)      tATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTa                                                                                 >  1:5497633/1‑100 (MQ=255)       ttGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTAt                                                                                >  1:4725435/2‑100 (MQ=255)       aTGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTAt                                                                                >  1:3937383/1‑100 (MQ=255)       aTGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTAt                                                                                >  1:4319056/1‑100 (MQ=255)        tGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATg                                                                               >  1:4915280/1‑100 (MQ=255)         gggTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGa                                                                              >  1:1201352/1‑100 (MQ=255)         gggTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGa                                                                              >  1:2478192/1‑100 (MQ=255)            gcATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATc                                                                           >  1:4215964/2‑100 (MQ=255)            atctGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATc                                                                           >  1:1703510/4‑100 (MQ=255)            acATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATc                                                                           >  1:4543037/2‑100 (MQ=255)            acATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATc                                                                           >  1:3949209/2‑100 (MQ=255)             cctGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCa                                                                          >  1:739882/3‑100 (MQ=255)             cATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCa                                                                          >  1:148833/1‑100 (MQ=255)             cATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCa                                                                          >  1:4587333/1‑100 (MQ=255)             cATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCa                                                                          >  1:5489045/1‑100 (MQ=255)             cATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCa                                                                          >  1:4486978/1‑100 (MQ=255)             cATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATAAATCa                                                                          >  1:56110/1‑100 (MQ=255)             agtGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCa                                                                          >  1:5053775/3‑100 (MQ=255)              ctGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCaa                                                                         >  1:1004042/2‑100 (MQ=255)              aTGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCaa                                                                         >  1:2201880/1‑100 (MQ=255)               tGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCaaa                                                                        >  1:343951/1‑100 (MQ=255)               tGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCaaa                                                                        >  1:3542530/1‑100 (MQ=255)               tGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCaaa                                                                        >  1:4722652/1‑100 (MQ=255)               tGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCaaa                                                                        >  1:4743648/1‑100 (MQ=255)                tgcagcaATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAAt                                                                       >  1:2509512/2‑100 (MQ=255)                ggCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAAt                                                                       >  1:5144046/1‑100 (MQ=255)                ggCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAAt                                                                       >  1:5321073/1‑100 (MQ=255)                  cagcaATTTTTAATAACACTAACGCGATTCCGGTTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGACTCAAATGc                                                                     >  1:1261549/1‑100 (MQ=255)                   ggcaATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCg                                                                    >  1:918523/2‑100 (MQ=255)                   agcaATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCg                                                                    >  1:2693087/1‑100 (MQ=255)                    gcaATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGt                                                                   >  1:3487775/1‑100 (MQ=255)                              tcaaCACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAatat                                                         >  1:4374812/3‑100 (MQ=255)                              ataaCACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAatat                                                         >  1:558757/1‑100 (MQ=255)                               taaCACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATAtt                                                        <  1:3756693/100‑1 (MQ=255)                                aaCACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTa                                                       >  1:1752263/1‑100 (MQ=255)                                   ccTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCa                                                    >  1:2895636/2‑100 (MQ=255)                                   acTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCa                                                    >  1:4610580/1‑100 (MQ=255)                                    ccgaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAt                                                   >  1:86194/4‑100 (MQ=255)                                    cTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAt                                                   >  1:4371902/1‑100 (MQ=255)                                    cTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAt                                                   >  1:2285832/1‑100 (MQ=255)                                    acaaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAt                                                   >  1:1823279/3‑100 (MQ=255)                                     cgaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAtt                                                  >  1:2221903/3‑100 (MQ=255)                                      aaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAttt                                                 >  1:4536541/1‑100 (MQ=255)                                      aaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAttt                                                 >  1:674108/1‑100 (MQ=255)                                      aaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAttt                                                 >  1:2066467/1‑100 (MQ=255)                                      aaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAttt                                                 >  1:3829309/1‑100 (MQ=255)                                      aaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCAttt                                                 >  1:2838041/1‑100 (MQ=255)                                      aaCGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGAGTTTAAAAATATTAGCAttt                                                 >  1:3344947/1‑100 (MQ=255)                                                cGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGttat                                       >  1:1823735/1‑100 (MQ=255)                                                cGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGttat                                       >  1:4743220/1‑100 (MQ=255)                                                cGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGttat                                       >  1:324520/1‑100 (MQ=255)                                                     ctcATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCa                                  >  1:2424404/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:261759/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:368563/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:576728/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:4923611/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:3090958/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:1503937/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:894660/1‑100 (MQ=255)                                                       cATTTTTATAGGGGCTTTAAAAGTACCATTATCAGGTTTGTTCTTTTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATg                                >  1:3172692/1‑100 (MQ=255)                                                              gtaGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTa                         >  1:4900418/2‑100 (MQ=255)                                                               taGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTAt                        >  1:2348361/1‑100 (MQ=255)                                                               taGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTAt                        >  1:3295087/1‑100 (MQ=255)                                                               taGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTAt                        >  1:1985586/1‑100 (MQ=255)                                                               taGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCCTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTAt                        >  1:3242589/1‑100 (MQ=255)                                                                aGGGGCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTAtc                       <  1:646443/100‑2 (MQ=255)                                                                  tggCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTgg                     >  1:2278951/2‑100 (MQ=255)                                                                  gggCTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTgg                     >  1:3025609/1‑100 (MQ=255)                                                                     cTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcgg                  <  1:2457298/100‑1 (MQ=255)                                                                     cTTTAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcga                  <  1:5292851/100‑2 (MQ=255)                                                                      tttAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcggg                 <  1:201453/100‑2 (MQ=255)                                                                      tttAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcggc                 <  1:5510547/100‑1 (MQ=255)                                                                       ttAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcggca                <  1:4593861/100‑2 (MQ=255)                                                                       ttAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGgcggcg                <  1:2948923/100‑1 (MQ=255)                                                                        tAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGt               >  1:3141127/1‑100 (MQ=255)                                                                        tAACAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGt               <  1:2856616/100‑1 (MQ=255)                                                                          catgccccATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcggccaga             >  1:613763/7‑96 (MQ=255)                                                                          aCAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTggcgtagatc             >  1:2653060/1‑94 (MQ=255)                                                                          aCAGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTag             <  1:1662299/100‑2 (MQ=255)                                                                            tgTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGgcggcgagatc           >  1:1158604/2‑95 (MQ=255)                                                                            aGTACCATTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGgcggcgagatc           >  1:2560991/1‑95 (MQ=255)                                                                                  aTTATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTa     >  1:1559616/1‑100 (MQ=255)                                                                                    tATCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTACt   <  1:125155/100‑1 (MQ=255)                                                                                     aTCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTACTa  >  1:2692925/1‑100 (MQ=255)                                                                                     aTCAGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTACTa  >  1:4937245/1‑100 (MQ=255)                                                                                        |                                                                                                 TGGATTATGGGTCATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTAACAGTACCATTATCGGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTACTA  >  NC_007793/277730‑277913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑