Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	240,358	T→A	N358K (AAT→AAA)	SAUSA300_RS01070 →	peptide ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	240,358	0	T	A	100.0%	50.4 / NA	18	N358K (AAT→AAA)	SAUSA300_RS01070	peptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/3);  total (15/3)

TGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAATCGATGCCTCG  >  NC_007793/240274‑240444                                                                                     |                                                                                       ataGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGAt                                                                         >  1:170533/3‑100 (MQ=255)              cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAATCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                            >  1:1757399/1‑100 (MQ=255)              cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                            >  1:5509703/1‑100 (MQ=255)              cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                            >  1:215438/1‑100 (MQ=255)                gTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAaat                                                          >  1:5348196/1‑100 (MQ=255)                     gTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAGGTAAAtaataa                                                     <  1:4248867/100‑1 (MQ=255)                                  cAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTAt                                        >  1:5347872/1‑100 (MQ=255)                                                    cgaTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATCACg                      >  1:105067/3‑100 (MQ=255)                                                     tATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTagac                     <  1:5025439/100‑4 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTGCGAc                    >  1:2009876/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGaa                    >  1:4219810/1‑99 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc                    >  1:1845138/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc                    >  1:2158557/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc                    >  1:3025859/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc                    >  1:471033/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc                    >  1:532493/1‑100 (MQ=255)                                                      aTGANAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc                    >  1:2867380/1‑100 (MQ=255)                                                                        cTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAATCGATGCCTCg  <  1:4897808/100‑1 (MQ=255)                                                                                     |                                                                                       TGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAATCGATGCCTCG  >  NC_007793/240274‑240444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑