Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	125,592	A→C	K28Q (AAA→CAA)	SAUSA300_RS00575 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	125,592	0	A	C	100.0%	111.4 / NA	38	K28Q (AAA→CAA)	SAUSA300_RS00575	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/6);  total (32/6)

AAGAGGCGATAGAATGTCTAAAATCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCAAAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAATAGCGCATT  >  NC_007793/125498‑125686                                                                                               |                                                                                               ctgagGCGATAGAATGTCTAAAATCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTa                                                                                           >  1:1104720/3‑100 (MQ=255)           aGAATGTCTAAAATCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTGCCAACCAAAg                                                                                 >  1:4607662/1‑100 (MQ=255)           aGAATGTCTAAAATCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAg                                                                                 >  1:2813645/1‑100 (MQ=255)                gTCTAAAATCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCt                                                                            <  1:5071912/100‑1 (MQ=255)                       ctCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCAtt                                                                     >  1:1146153/2‑100 (MQ=255)                        tCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATta                                                                    >  1:5181638/1‑100 (MQ=255)                        tCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATta                                                                    >  1:2151244/1‑100 (MQ=255)                         cAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATtat                                                                   >  1:1130235/1‑100 (MQ=255)                                 ttACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGc                                                           <  1:4382420/100‑1 (MQ=255)                                 ttACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGc                                                           <  1:2594880/100‑1 (MQ=255)                                      atatTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAgggg                                                      <  1:2204130/100‑1 (MQ=255)                                                 aCTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGagatcggaaga                                           >  1:3882556/1‑90 (MQ=255)                                                 aCTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAAt                                           >  1:2028060/1‑100 (MQ=255)                                                           gtAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATa                                 >  1:2764236/2‑100 (MQ=255)                                                           accggtATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATa                                 >  1:1204367/6‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATATTTTAGCAGGGGATGCTGATAATTTTGCTGATaa                                >  1:5181217/1‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaa                                >  1:2197503/1‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaa                                >  1:3993219/1‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaa                                >  1:560991/1‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaa                                >  1:4528487/1‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaa                                >  1:5075750/1‑100 (MQ=255)                                                            tAGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTGGCTGATaa                                >  1:1724653/1‑100 (MQ=255)                                                             aGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaaa                               >  1:1866429/1‑100 (MQ=255)                                                             aGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaaa                               >  1:5425084/1‑100 (MQ=255)                                                             aGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaaa                               >  1:5298255/1‑100 (MQ=255)                                                             aGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaaa                               >  1:1863201/1‑100 (MQ=255)                                                             aGCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATaaa                               >  1:1136093/1‑100 (MQ=255)                                                              gCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAAt                              >  1:1958616/1‑100 (MQ=255)                                                              gCTATGATGTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCCATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAAt                              >  1:2887599/1‑100 (MQ=255)                                                                 atgatgTGCTATACAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATggg                           >  1:75292/1‑100 (MQ=255)                                                                           ataCAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCtttt                 >  1:1437251/1‑100 (MQ=255)                                                                             aCAGTAGTGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGa               >  1:3631453/1‑100 (MQ=255)                                                                                  agtGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAAt          >  1:41471/1‑100 (MQ=255)                                                                                  agtGACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAAt          >  1:2792452/1‑100 (MQ=255)                                                                                     gACCTATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAATAgc       >  1:4373625/1‑100 (MQ=255)                                                                                        cTATCCACGATTACCTACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAATAGCGCa    <  1:4298440/100‑1 (MQ=255)                                                                                         tATCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAATAGCGCAt   <  1:4346763/100‑1 (MQ=255)                                                                                          aTCCACAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAATAGCGCAtt  >  1:914114/1‑100 (MQ=255)                                                                                               |                                                                                               AAGAGGCGATAGAATGTCTAAAATCAGGTCTTTTACAATATTAAGTCTACTTATTTACTTAGCTATGATGTGCTATACAGTAGTGACCTATCCAAAATTACCAACCAAAGTGCCTATTCATTATAATTTAGCAGGGGATGCTGATAATTTTGCTGATAAATGGGTGCTGCTTTTGATTAATAGCGCATT  >  NC_007793/125498‑125686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑