Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,606,040	C→T	E109K (GAA→AAA)	SAUSA300_RS13395 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,606,040	0	C	T	100.0%	19.1 / NA	9	E109K (GAA→AAA)	SAUSA300_RS13395	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/7);  total (2/7)

TTATATCATCTGGTGATTTTAACTGATTGGCTGGCTTTAGTAACTCGCTTAATTTTTCACTTTCTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATCACAGC  >  NC_007793/2605977‑2606136                                                                |                                                                                                 cgatcttgtcTGGTGATTTGGTTTGATGTACTGGTTTTAATAACGTGCTTAAATTTTTACTTTTGATTAAACTTCCACCAAATTGATAATTATGTTCTgg                                                              <  1:4014510/92‑1 (MQ=38)                                                   gctccttCACTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAAATATCTTTTTTGTTTTCATTTATGTAACCtt            >  1:4563431/6‑100 (MQ=18)                                                   actccttCACTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCtt            >  1:5365218/6‑100 (MQ=255)                                                     tccttCACTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCa          <  1:5113024/97‑1 (MQ=255)                                                          tgcTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATcac     <  1:927943/98‑1 (MQ=255)                                                          cACTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATcac     <  1:1071598/100‑1 (MQ=255)                                                          cACTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATcac     <  1:4124329/100‑1 (MQ=255)                                                          cACTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATcac     <  1:4164058/100‑1 (MQ=255)                                                            cTTTTTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATcacac   <  1:2812723/100‑2 (MQ=255)                                                             ttttTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATCACAga  <  1:4699757/100‑2 (MQ=255)                                                             ttttTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATCACAGc  <  1:3248411/100‑1 (MQ=255)                                                             ttttTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATCACAGc  <  1:3528108/100‑1 (MQ=255)                                                             ttttTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATCACAGc  <  1:5006123/100‑1 (MQ=255)                                                             ttttTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCCTCAATCACAGc  <  1:936431/100‑1 (MQ=255)                                                                |                                                                                                 TTATATCATCTGGTGATTTTAACTGATTGGCTGGCTTTAGTAACTCGCTTAATTTTTCACTTTCTATCATAGCACCACCAAATTGATAATTATGTTCTGGTGTAGCATAAGCAGTAAAATTATCTTTTTTGTTTTCATTTATGTAACCTTCAATCACAGC  >  NC_007793/2605977‑2606136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑