Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,557,059	G→A	A220T (GCA→ACA)	SAUSA300_RS13150 →	glycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,557,059	0	G	A	100.0%	50.0 / NA	19	A220T (GCA→ACA)	SAUSA300_RS13150	glycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/8);  total (11/8)

AAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATACCAGGTTCTG  >  NC_007793/2556963‑2557155                                                                                                 |                                                                                                 aaaGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACaa                                                                                               <  1:1229981/100‑1 (MQ=255) aaaGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAATCCTTTATTGGGCGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACaa                                                                                               <  1:3558388/100‑1 (MQ=255)                  aGTGGCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTg                                                                              >  1:5473177/1‑100 (MQ=255)                   gtagCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTgg                                                                             >  1:4619892/4‑100 (MQ=255)                   atGGCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTgg                                                                             >  1:3607332/2‑100 (MQ=255)                     tgCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAgatcgga                                                                           >  1:2485907/2‑94 (MQ=255)                          ttgATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGc                                                                      >  1:1297038/2‑100 (MQ=255)                          gtgATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGc                                                                      >  1:3560500/1‑100 (MQ=255)                                           ggcctGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATg                                                     >  1:414996/5‑100 (MQ=255)                                              ttGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATgata                                                  <  1:5469077/100‑1 (MQ=255)                                                 ggTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATgataaga                                               >  1:1004773/1‑100 (MQ=255)                                                             gCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGca                                   >  1:310844/1‑100 (MQ=255)                                                                                      aaGGCGCTGATACATAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATAcc          <  1:2837889/100‑1 (MQ=255)                                                                                      aaGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATAcc          <  1:2957173/100‑1 (MQ=255)                                                                                      aaGGCGCTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATAcc          <  1:2007990/100‑1 (MQ=255)                                                                                          cgcTGATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATACCAGGt      <  1:4662275/100‑1 (MQ=255)                                                                                              gATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATACCAGGTTCTg  <  1:4015288/100‑1 (MQ=255)                                                                                              gATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATACCAGGTTCTg  >  1:5452569/1‑100 (MQ=255)                                                                                              gATACAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATACCAGGTTCTg  >  1:1717359/1‑100 (MQ=255)                                                                                                 |                                                                                                 AAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAATCCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCAAAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGCACAGGAAAGTCCGTTAATCAAATACCAGGTTCTG  >  NC_007793/2556963‑2557155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑