Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,532,090	A→T	intergenic (‑241/‑14)	SAUSA300_RS13005 ← / → SAUSA300_RS13010	hypothetical protein/methicillin resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,532,090	0	A	T	100.0%	41.5 / NA	17	intergenic (‑241/‑14)	SAUSA300_RS13005/SAUSA300_RS13010	hypothetical protein/methicillin resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/1);  total (16/1)

ATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCT  >  NC_007793/2532010‑2532181                                                                                 |                                                                                            aTGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTAATAATAAATTCAAAAATTATAGTTCAATTCAGAGTgagatcggaagagcacacg                                                                          >  1:1890321/1‑90 (MQ=255)     gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTg                                                                      >  1:2798984/1‑100 (MQ=255)                                     caTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGttt                                      >  1:3533288/2‑100 (MQ=255)                                     aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGttt                                      <  1:1942347/100‑1 (MQ=255)                                      ccaGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:2743736/3‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:2311854/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:1266694/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:1981683/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:310118/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:1940436/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:3695327/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:5158710/1‑100 (MQ=255)                                      aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTa                                     >  1:932857/1‑100 (MQ=255)                                        aGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTaca                                   >  1:2072315/1‑100 (MQ=255)                                                                 ataGTTCAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTacacac          >  1:4407906/1‑100 (MQ=255)                                                                       cAATTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAg    >  1:2326223/1‑100 (MQ=255)                                                                         aTTCAGAGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCt  >  1:392785/1‑100 (MQ=255)                                                                                 |                                                                                            ATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCT  >  NC_007793/2532010‑2532181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑