Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,521,979	A→C	L32V (TTG→GTG)	SAUSA300_RS12945 ←	nitrite reductase NAD(P)H small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,521,979	0	A	C	100.0%	21.5 / NA	9	L32V (TTG→GTG)	SAUSA300_RS12945	nitrite reductase NAD(P)H small subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/9);  total (0/9)

TCCCCACTCACTGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACAACCCTACCTCTTTGCCTTTGACAATAACCTTTTTTCCAATTAGGGGTGTTAATTCATCTATAGTTGTC  >  NC_007793/2521888‑2522046                                                                                            |                                                                    tCCCCACTCACTGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACct                                                             <  1:3911596/100‑1 (MQ=255)  ccccACTCACTGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTAccac                                                            <  1:1484988/100‑3 (MQ=255)         cactGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCt                                                     <  1:2028938/100‑1 (MQ=255)          actGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCta                                                    <  1:3962474/100‑2 (MQ=255)           ctGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCttt                                                   <  1:881893/100‑1 (MQ=255)            tGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCTTTg                                                  <  1:1489643/100‑1 (MQ=255)                                 ttgtttgtGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCTTTGACAATAACCTTTTTTCCAAtt                             <  1:2115927/100‑1 (MQ=255)                                                            cGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCTTTGACAATAACCTTTTTTCCAATTAGGGGTGTTAATTCATCTATAGTTGTc  <  1:1632466/100‑1 (MQ=255)                                                            cGCATGAATTTTGCCACTTTCTGTTAAAAACACCCCTACCTCTTTGCCTTTGACAATAACCTTTTTTCCAATTAGGGGTGTTAATTCATCTATAGTTGTc  <  1:3571792/100‑1 (MQ=255)                                                                                            |                                                                    TCCCCACTCACTGTCCCTTCAGACAATGGTCCTTGTTTGTGTGGACAGATATTGTGAATCGCATGAATTTTGCCACTTTCTGTTAAAAACAACCCTACCTCTTTGCCTTTGACAATAACCTTTTTTCCAATTAGGGGTGTTAATTCATCTATAGTTGTC  >  NC_007793/2521888‑2522046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑