Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,249,855	G→T	I117I (ATC→ATA)	SAUSA300_RS11485 ←	ATP‑grasp domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,249,855	0	G	T	100.0%	73.8 / NA	24	I117I (ATC→ATA)	SAUSA300_RS11485	ATP‑grasp domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (2/22);  total (2/22)

AGCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAAGATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTA  >  NC_007793/2249810‑2249932                                              |                                                                              aGCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGcc                         <  1:5540109/100‑1 (MQ=255) aGCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGcc                         <  1:4798944/100‑1 (MQ=255)  gCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGccc                        <  1:326361/100‑2 (MQ=255)  gCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGccc                        <  1:4135249/100‑2 (MQ=255)  gCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCa                        <  1:4934358/100‑1 (MQ=255)   caacaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCaa                       <  1:906621/100‑1 (MQ=255)   caacaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCaa                       >  1:2885912/1‑100 (MQ=255)   caacaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCaa                       <  1:3431744/100‑1 (MQ=255)   caacaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCaa                       <  1:4739490/100‑1 (MQ=255)    aacaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCaag                      <  1:5257523/100‑2 (MQ=255)    aacaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAAt                      <  1:1739413/100‑1 (MQ=255)     acaaATAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAAtt                     <  1:5431432/100‑1 (MQ=255)        aaTAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGtt                  <  1:289179/100‑1 (MQ=255)          tAACGTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTg                <  1:835390/100‑1 (MQ=255)              gTTTTATCTAATGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCa            <  1:995818/100‑1 (MQ=255)                       caTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGt   >  1:4113019/2‑100 (MQ=255)                       aaTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGt   <  1:1195634/100‑1 (MQ=255)                        aTGCCTAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTACTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:1674793/100‑1 (MQ=255)                        aTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:5505452/100‑1 (MQ=255)                        aTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:577247/100‑1 (MQ=255)                        aTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:64770/100‑1 (MQ=255)                        aTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:832142/100‑1 (MQ=255)                        aTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:1317346/100‑1 (MQ=255)                        aTGCATAATATTGCTGATTCAATATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTa  <  1:1020847/100‑1 (MQ=255)                                              |                                                                              AGCAACAAATAACGTTTTATCTAATGCATAATATTGCTGATTCAAGATTGTTTCATCATGAATATATTGAAAATAAATCTTTTTATTTTCCTTATGTGCCAATTGTTTGATCAAGTTTTCGTA  >  NC_007793/2249810‑2249932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑