Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,089,184	C→G	G227R (GGT→CGT)	SAUSA300_RS10545 ←	amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,089,184	0	C	G	100.0%	69.8 / NA	25	G227R (GGT→CGT)	SAUSA300_RS10545	amidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/1);  total (24/1)

AACCTAAGAATTAAAACACTTCTTTCACAATCAATCTCTCATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACCATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTCCAACATCGAATCTATAGTTAGTACGATTATCACCGTA  >  NC_007793/2089097‑2089274                                                                                        |                                                                                           aaCCTAAGAATTAAAACACTTCTTTCACAATCAATCTCTCATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTc                                                                                >  1:2525346/1‑100 (MQ=255)                            caatcaatGTCTCATGCCATATCCACTCATTATCATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTATTGTTCCAGgcgg                                                     <  1:4255186/100‑2 (MQ=255)                                      ctctTGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:4805567/1‑100 (MQ=255)                                      ctcATTCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:1354505/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGgcgcagatcggaag                                           >  1:4426436/1‑90 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:4408460/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:955740/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:677256/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:673889/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:5499672/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:1315654/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:4683065/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:3587158/1‑100 (MQ=255)                                      ctcATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGcc                                           >  1:2700806/1‑100 (MQ=255)                                        cATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTg                                         >  1:1856803/1‑100 (MQ=255)                                        cATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTg                                         >  1:3301031/1‑100 (MQ=255)                                        cATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTg                                         >  1:300572/1‑100 (MQ=255)                                         aTGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGt                                        >  1:4388904/1‑100 (MQ=255)                                         aTGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGt                                        >  1:2526489/1‑100 (MQ=255)                                          tGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTc                                       >  1:4432656/1‑100 (MQ=255)                                          tGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTc                                       >  1:4511302/1‑100 (MQ=255)                                           gCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTcc                                      >  1:1772038/1‑100 (MQ=255)                                                atCCACTCATTATGATTGTTCCAATAAATGCGACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTCCAACAt                                 >  1:3908645/1‑100 (MQ=255)                                                                       taatgctcGACTCCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTCCAACATCGAATCTATAGTTAGTACGATTa          >  1:3751991/8‑100 (MQ=255)                                                                               gACACCAACGATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTCCAACATCGAATCTATAGTTAGTACGATTATCACCGTa  >  1:4706696/1‑100 (MQ=255)                                                                                        |                                                                                           AACCTAAGAATTAAAACACTTCTTTCACAATCAATCTCTCATGCCATATCCACTCATTATGATTGTTCCAATAAATGCGACACCAACCATCTATAATTTCAAACACATATATTAATGTTCCAGGCGCGTATACAGCCTGTCCAACATCGAATCTATAGTTAGTACGATTATCACCGTA  >  NC_007793/2089097‑2089274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑