Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,000,294	A→G	noncoding (404/2926 nt)	SAUSA300_RS10040 ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,000,294	0	A	G	100.0%	18.0 / NA	15	noncoding (404/2926 nt)	SAUSA300_RS10040	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/0);  total (15/0)

TCACTATCGGTCACTAGAGAGTATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATGTCG  >  NC_007793/2000198‑2000367                                                                                                 |                                                                          tCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTTAggag                                                                        <  1:1255614/100‑1 (MQ=11)        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCGGgagagatcgg                                                                 >  1:559528/1‑96 (MQ=11)           ccACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagt                                                              >  1:1302916/2‑93 (MQ=11)                       aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGACCCACTCAGGAGAGAACGAAGTTTTGACTACAgg                                                  >  1:3966395/1‑100 (MQ=11)                         ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc                                                >  1:1188375/1‑98 (MQ=11)                         ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc                                                >  1:2052484/1‑98 (MQ=11)                         ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc                                                >  1:5207377/1‑98 (MQ=11)                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                               >  1:1831766/1‑97 (MQ=11)                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                               >  1:1987959/1‑97 (MQ=11)                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                               >  1:587029/1‑97 (MQ=11)                           cgCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg                                              >  1:4134688/2‑96 (MQ=11)                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg                                              >  1:2431906/1‑96 (MQ=11)                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg                                              >  1:4082830/1‑96 (MQ=11)                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggcag                                              >  1:859698/1‑96 (MQ=11)                           aGCCTTGGGAGATGGGCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg                                              >  1:1710462/1‑96 (MQ=11)                                                                       gtTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATgtcg  >  1:4939747/1‑100 (MQ=2)                                                                                                 |                                                                          TCACTATCGGTCACTAGAGAGTATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATGTCG  >  NC_007793/2000198‑2000367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑