Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,000,230 A→G noncoding (468/2926 nt) SAUSA300_RS10040 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,000,2300AG83.0% 41.8 / 29.4 56noncoding (468/2926 nt)SAUSA300_RS1004023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/3);  major base G (41/3);  minor base . (9/0);  total (50/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-05

CTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTA  >  NC_007793/2000170‑2000323
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cttccgatcTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACgtgt                                                        <  1:1806409/92‑1 (MQ=2)
cTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTCTTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACgtgt                                                        <  1:4224143/100‑1 (MQ=11)
cTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACgtgt                                                        <  1:3222117/100‑1 (MQ=11)
  tttCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACgtgtcc                                                      >  1:906273/1‑98 (MQ=2)
  tttCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACgtgtcc                                                      >  1:2495870/1‑98 (MQ=2)
    acACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCGCGTGTTCcg                                                    >  1:1627662/2‑100 (MQ=11)
     cgccTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCcgc                                                   >  1:3006003/3‑99 (MQ=2)
         tttCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCcgcctta                                               <  1:4081795/100‑6 (MQ=2)
           ccccTCACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACt                                             >  1:435423/2‑100 (MQ=2)
              tccACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAg                                          >  1:2421894/3‑100 (MQ=11)
              cTCACGGTACTGGTTCACTATCAGTC‑‑‑‑AG‑GAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAcagatcgga                                      >  1:3323828/1‑92 (MQ=2)
               tCACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAgg                                         >  1:2821083/1‑100 (MQ=11)
                taCGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAT‑GATGGTCCCCCCATATTGAGACAGGATAGCACGTGTCCCGCCCTACTCatcg                                        >  1:2292046/2‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAcaagatc                                        >  1:305452/1‑94 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTT‑GGAGGATGGTCCCCTCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcg                                        >  1:4201089/1‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGTCCTAcagatcg                                        >  1:2734698/1‑94 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAcaagatc                                        >  1:5038482/1‑94 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcg                                        >  1:5395560/1‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcg                                        >  1:3619821/1‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCACCCTACTCatcg                                        >  1:755000/1‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAcacaacg                                        >  1:5427497/1‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACtagatc                                        >  1:2102161/1‑95 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACtagatc                                        >  1:2921799/1‑95 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCAGT‑CAGGAGAATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcg                                        >  1:503572/1‑97 (MQ=2)
                cACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCTGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAggg                                        >  1:492853/1‑99 (MQ=11)
                cACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGa                                        >  1:3508281/1‑100 (MQ=11)
                cACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGa                                        >  1:1209161/1‑100 (MQ=11)
                cACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGa                                        >  1:4165811/1‑100 (MQ=11)
                cACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGa                                        >  1:4237151/1‑100 (MQ=11)
                cACGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATACCGACGGAATTTCACGTGTTCCGGCGTACTCAGGa                                        >  1:2365851/1‑100 (MQ=11)
                 aCGGTACTGGTTCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                       >  1:2161045/1‑100 (MQ=11)
                  aggTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgag                                      >  1:4398470/2‑95 (MQ=2)
                   cgatcTGGTTTACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTAATCAGGATcc                                     <  1:1913881/96‑1 (MQ=255)
                   agTACTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagc                                     >  1:3481676/2‑94 (MQ=2)
                    gTACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagca                                    >  1:4082565/1‑93 (MQ=2)
                    ctACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagct                                    >  1:3120111/2‑93 (MQ=2)
                    ccaCTGGTTCACTATCGGTCAGTCAG‑GAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagct                                    >  1:3353365/3‑93 (MQ=2)
                     tACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagctc                                   >  1:2384307/1‑92 (MQ=2)
                     tACTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagctc                                   >  1:730249/1‑92 (MQ=2)
                      gcTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagctca                                  >  1:4640889/2‑91 (MQ=2)
                      aCTGGTTCACTATCGGTCAGT‑CAGGAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCatcgagctca                                  >  1:3099935/1‑91 (MQ=2)
                            tCACTATCGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTTAggag                            <  1:1255614/100‑1 (MQ=11)
                                   cGGTCACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCGGgagagatcgg                     >  1:559528/1‑96 (MQ=11)
                                      ccACT‑AGGGAGTATTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagt                  >  1:1302916/2‑93 (MQ=11)
                                                   aTTTAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGACCCACTCAGGAGAGAACGAAGTTTTGACTACAgg      >  1:3966395/1‑100 (MQ=11)
                                                     ttAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc    >  1:1188375/1‑98 (MQ=11)
                                                     ttAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc    >  1:5207377/1‑98 (MQ=11)
                                                     ttAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc    >  1:2052484/1‑98 (MQ=11)
                                                      tAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct   >  1:1831766/1‑97 (MQ=11)
                                                      tAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct   >  1:1987959/1‑97 (MQ=11)
                                                      tAGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct   >  1:587029/1‑97 (MQ=11)
                                                       cgCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg  >  1:4134688/2‑96 (MQ=11)
                                                       aGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg  >  1:4082830/1‑96 (MQ=11)
                                                       aGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg  >  1:2431906/1‑96 (MQ=11)
                                                       aGCCTTGGGA‑GATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggcag  >  1:859698/1‑96 (MQ=11)
                                                       aGCCTTGGGA‑GATGGGCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg  >  1:1710462/1‑96 (MQ=11)
                                                             |                                                                                              
CTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTA  >  NC_007793/2000170‑2000323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑