Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	61,025	G→A	L62F (CTT→TTT)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	61,025	0	G	A	100.0%	94.8 / NA	32	L62F (CTT→TTT)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/8);  total (24/8)

ATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAC  >  NC_007793/60934‑61121                                                                                             |                                                                                                 atTAAACGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAACACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAAccc                                                                                          <  1:4225625/100‑1 (MQ=255)  ttAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCa                                                                                         <  1:1406015/100‑1 (MQ=255)    aaaaGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAg                                                                                       <  1:892424/100‑1 (MQ=255)    aaaaGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAg                                                                                       <  1:1371333/100‑1 (MQ=255)       aGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAgatc                                                                                    >  1:552277/1‑97 (MQ=255)           tGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAgatcggaa                                                                                >  1:1210744/1‑93 (MQ=255)            gCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAGTCataata                                                                               >  1:1609846/1‑100 (MQ=255)                                           ttATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTg                                                <  1:2422354/100‑1 (MQ=255)                                                  aGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTgg                                         >  1:1341450/1‑100 (MQ=255)                                                                   tgctcttccgaTCTC‑CCTATAAAAAAACTAACCCAAGACATAATCTGATTAATGAATGCTTTTTTGCCATGTTATGATGTTGGTTCATCAGCAAGTATcc                        <  1:4567276/90‑1 (MQ=255)                                                                      aatGCCAATCTA‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATa                     >  1:2413142/1‑100 (MQ=255)                                                                      aatGCCAATCTA‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATa                     <  1:3095498/100‑1 (MQ=255)                                                                        ttccgaTCTA‑CCTGTAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACa                   <  1:2468101/95‑1 (MQ=255)                                                                         tccgaTCTACCCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACa                   <  1:2414114/96‑1 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATGCATACAAATAAAGGt          >  1:4565349/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:3478256/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:3264819/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:3859488/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:4307888/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:472207/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:4785607/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:126930/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:1066404/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:328619/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:2934181/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:2800950/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:2491444/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:2430906/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:2247724/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:218079/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:214576/1‑100 (MQ=255)                                                                                 a‑CCTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCCTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:5087224/1‑100 (MQ=255)                                                                                  ggcTATAAAAAAACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGt          >  1:3281577/3‑100 (MQ=255)                                                                                          aaaaACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc  <  1:2891381/100‑1 (MQ=255)                                                                                             |                                                                                                 ATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCTA‑CCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAC  >  NC_007793/60934‑61121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑