Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,961,354	C→T	noncoding (9/74 nt)	SAUSA300_RS09735 ←	tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,961,354	0	C	T	100.0%	183.6 / NA	61	noncoding (9/74 nt)	SAUSA300_RS09735	tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/60);  total (1/60)

ACGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCAAGAACTTTTTGAATTTAATTTAAATGTTATCT  >  NC_007793/1961260‑1961449                                                                                               |                                                                                                aCGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATccc                                                                                            <  1:1137577/100‑1 (MQ=255) aCGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATccc                                                                                            <  1:1315652/100‑1 (MQ=255) aCGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATccc                                                                                            <  1:799748/100‑1 (MQ=255) aCGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATccc                                                                                            <  1:5289577/100‑1 (MQ=255)  cGAGACCTATTAAATT‑AAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgc                                                                                          <  1:3932420/100‑1 (MQ=255)   gagaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATccccg                                                                                          <  1:2520294/100‑3 (MQ=255)   gagaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATcccag                                                                                          <  1:4972950/100‑3 (MQ=255)    tgaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                                         <  1:643601/99‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgct                                                                                         <  1:3996191/100‑2 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                                         <  1:485100/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                                         <  1:3911480/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                                         <  1:4195652/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcg                                                                                         <  1:4654142/100‑1 (MQ=255)    agaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgca                                                                                         <  1:3220863/100‑2 (MQ=255)     gaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcgg                                                                                        <  1:1472805/100‑2 (MQ=255)     gaCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcgg                                                                                        <  1:2667766/100‑2 (MQ=255)      aCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCt                                                                                       <  1:405847/100‑1 (MQ=255)      aCCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCt                                                                                       <  1:2761373/100‑1 (MQ=255)      aCCTATTAAATTAAAAACTATGTATTGCGGGAGGCCGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCt                                                                                       <  1:2365990/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcgggt                                                                                      <  1:2685776/100‑4 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCcgcgggt                                                                                      <  1:2877705/100‑4 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                                      <  1:3793906/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                                      <  1:947483/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                                      <  1:2303671/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                                      <  1:2695292/100‑1 (MQ=255)       ccTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTa                                                                                      <  1:2731041/100‑1 (MQ=255)        cTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCtca                                                                                     <  1:546259/100‑3 (MQ=255)        cTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTag                                                                                     <  1:3609516/100‑2 (MQ=255)        cTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTaa                                                                                     <  1:1366565/100‑1 (MQ=255)         tATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCgcgcggtg                                                                                    <  1:732840/100‑5 (MQ=255)         tATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAat                                                                                    <  1:3379206/100‑1 (MQ=255)              aattaaaAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAAtattat                                                                               <  1:4069916/100‑1 (MQ=255)                  aaaaaCTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTg                                                                           <  1:2007199/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAAttcc                                                                     <  1:3418371/100‑3 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                                     <  1:4879452/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                                     <  1:2551521/100‑1 (MQ=255)                        tATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAc                                                                     <  1:790701/100‑1 (MQ=255)                         aTGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAct                                                                    <  1:758536/100‑2 (MQ=255)                          tGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAcgg                                                                   <  1:4359414/100‑3 (MQ=255)                          tGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAcag                                                                   <  1:3700483/100‑3 (MQ=255)                          tGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCt                                                                   <  1:4534088/100‑1 (MQ=255)                            tATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAccccc                                                                 <  1:1720933/100‑4 (MQ=255)                            tATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATAAcggga                                                                 <  1:2913446/100‑5 (MQ=17)                             aTTGCGGGAGGCGGATTTGAACCACCGCCCTTCGGGTTATGAGCCCGACGAGCTACCGAGCTGCTCTATCCCGCGCTAATATTATTTTGAATTAcagggc                                                                <  1:604109/100‑6 (MQ=17)                              ttGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTAcccacta                                                               <  1:2598070/100‑6 (MQ=255)                              ttGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCtaatt                                                               <  1:1030337/100‑1 (MQ=255)                                      ggCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATAcc                                                       <  1:3699890/100‑1 (MQ=255)                                                    accGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAAcca                                         <  1:1062306/100‑2 (MQ=255)                                                    accGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCt                                         <  1:474985/100‑1 (MQ=255)                                                     ccGATCTTGGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTa                                        <  1:3665943/100‑1 (MQ=255)                                                      gatcttccGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTaa                                       <  1:921169/93‑1 (MQ=255)                                                           tttcggTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa                                  <  1:1630067/96‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa                                  <  1:4867783/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa                                  <  1:3789391/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa                                  <  1:722404/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa                                  <  1:225320/100‑1 (MQ=255)                                                           ttCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACATACAGTCa                                  <  1:1338440/100‑1 (MQ=255)                                                           ttCGGGCTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCa                                  <  1:3788622/100‑1 (MQ=255)                                                            acGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCaa                                 <  1:338485/99‑1 (MQ=255)                                                             cGGGTTATGAGCCCGACGAGCTACCGAACTGCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCaat                                <  1:4416796/100‑2 (MQ=255)                                                                                           gCTCTATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGCCAAGAACTTTTTGAATTTAATTTAAATGTTAtct  >  1:3202669/1‑100 (MQ=255)                                                                                               |                                                                                                ACGAGACCTATTAAATTAAAAACTATGTATTGCGGGAGGCGGATTTGAACCACCGACCTTCGGGTTATGAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGCTAATATTATTTTGAATTACCTAATTAATATACCATAATCAAAAACCTAAAGTCAAGAACTTTTTGAATTTAATTTAAATGTTATCT  >  NC_007793/1961260‑1961449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑