Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,944,161	A→G	L55P (CTC→CCC)	SAUSA300_RS09625 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,944,161	0	A	G	87.5%	11.5 / ‑4.0	8	L55P (CTC→CCC)	SAUSA300_RS09625	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (5/2);  total (6/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACATGTATGTATGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGAGATCAGATTTTGCGATTTTGCCTTGGATAGAG  >  NC_007793/1944064‑1944193                                                                                                   |                                 aaaCATGTATGTATGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑Gggga                                >  1:115381/1‑100 (MQ=255)   aCATGTATGTATGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGGATc                              <  1:2937664/100‑1 (MQ=255)      tgtatgtaTGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGGATCAga                           >  1:2083539/1‑100 (MQ=255)       gtatgtatGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTCGGGAGATCGga                           >  1:220529/1‑100 (MQ=255)       gtatgtatGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGGATCAgat                          >  1:1362480/1‑100 (MQ=255)             atgAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGGATCAgattttgcg                    <  1:4489456/100‑1 (MQ=255)              tgAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGAATCAgatcggaaga                   >  1:1120867/1‑93 (MQ=255)                               aGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGGATCAGATTTTGCGATTTTGCCTTgagatcgg  >  1:1363892/1‑93 (MQ=255)                               aGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGGGATCAGATTTTGCGATTTTGCCTTGGATagag  >  1:14473/1‑100 (MQ=255)                                                                                                   |                                 AAACATGTATGTATGAGTATATTTGGAACTAGTAATGTATTGTTAAATATTGAAGATGATTGAATTATGGAATTTTTTTCGAGATATGTTATTTC‑GGAGATCAGATTTTGCGATTTTGCCTTGGATAGAG  >  NC_007793/1944064‑1944193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑