Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,503,575	T→G	R65R (AGG→CGG)	SAUSA300_RS07300 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,503,575	0	T	G	100.0%	176.3 / NA	61	R65R (AGG→CGG)	SAUSA300_RS07300	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/61);  total (0/61)

CTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCTTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAC  >  NC_007793/1503478‑1503665                                                                                                  |                                                                                           cTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTg                                                                                          <  1:1366844/100‑1 (MQ=255) cTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTg                                                                                          <  1:4066031/100‑1 (MQ=255) cTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTg                                                                                          <  1:3560744/100‑1 (MQ=255) cTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTg                                                                                          <  1:4105323/100‑1 (MQ=255) cTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTg                                                                                          <  1:5020883/100‑1 (MQ=255)  ttttAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTgg                                                                                         <  1:4422401/100‑2 (MQ=255)  ttttAAGCTTTTCTATAAATAACTTTCGACTTGCTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGa                                                                                         <  1:5511655/100‑1 (MQ=255)   tttAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTgga                                                                                        <  1:479459/100‑3 (MQ=255)    ttAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTgggt                                                                                       <  1:684138/100‑4 (MQ=255)    ttAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTgggt                                                                                       <  1:1876326/100‑4 (MQ=255)                   aataaCTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACtt                                                                        <  1:838146/100‑1 (MQ=255)                       aCTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCtat                                                                    <  1:4021312/100‑1 (MQ=255)                       aCTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCtat                                                                    <  1:3086525/100‑1 (MQ=255)                                   tttgCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACg                                                        <  1:86531/97‑1 (MQ=255)                                       cTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGt                                                    <  1:5292625/100‑1 (MQ=255)                                              tGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTagccgtc                                             <  1:2369673/100‑7 (MQ=255)                                                         aGACAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGt                                  <  1:1454029/100‑1 (MQ=255)                                                            aaGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTTCTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATg                               <  1:4528092/100‑1 (MQ=255)                                                             aGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGc                              <  1:5248454/100‑1 (MQ=255)                                                                          tAGTAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:2292657/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:4934076/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:4791725/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:4532267/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:2138947/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:3287440/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:5469128/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:592760/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:3880582/100‑1 (MQ=255)                                                                          tAGCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTc                 <  1:963143/100‑1 (MQ=255)                                                                            gCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCac               <  1:1953307/100‑2 (MQ=255)                                                                            gCAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAg               <  1:1070218/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAgc              <  1:879203/100‑2 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:391971/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:387081/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:2493488/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:4268473/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:2618500/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:5188761/100‑1 (MQ=255)                                                                             cAATCAGTAGCTCAAATTTCCGTGAAATCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGt              <  1:2715036/100‑1 (MQ=255)                                                                              aaTCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAgcc             <  1:541944/100‑3 (MQ=255)                                                                               aTCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGtat            <  1:1175832/100‑1 (MQ=255)                                                                               aTCAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGtat            <  1:3988944/100‑1 (MQ=255)                                                                                 cAGTAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTatat          <  1:2824138/100‑1 (MQ=255)                                                                                    tAGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGc       <  1:2917910/100‑1 (MQ=255)                                                                                     aGCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCt      <  1:1778577/100‑1 (MQ=255)                                                                                      gCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCtt     <  1:4099781/100‑1 (MQ=255)                                                                                      gCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCtt     <  1:1269749/100‑1 (MQ=255)                                                                                      gCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCtt     <  1:4926024/100‑1 (MQ=255)                                                                                      gCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCtt     <  1:200547/100‑1 (MQ=255)                                                                                      gCTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCtt     <  1:3359483/100‑1 (MQ=255)                                                                                       cTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCttt    <  1:1012402/100‑1 (MQ=255)                                                                                       cTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCttt    <  1:3510216/100‑1 (MQ=255)                                                                                       cTCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTCGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCttg    <  1:3245653/100‑2 (MQ=255)                                                                                        tCAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCttag   <  1:4615054/100‑3 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCtttcc  <  1:530346/100‑3 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:2688113/100‑1 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:2718060/100‑1 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:4648357/100‑1 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:904804/100‑1 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCACTGAGCATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:1678137/100‑1 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGCAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:4477834/100‑1 (MQ=255)                                                                                         cAAATTTCCGTGAATTCATATAAGGTACTTCTATAATAAATTAACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAc  <  1:2032994/100‑1 (MQ=255)                                                                                                  |                                                                                           CTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTAGCTCAAATTTCCTTGAATTCATATAAGGTACTTCTATAATAAATTCACGATGTAATTTGATCTCATTGAGTATGCTATCAATATGTTCAGTATATGGCTTTAC  >  NC_007793/1503478‑1503665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑