Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,363,207	A→T	I761F (ATT→TTT)	SAUSA300_RS06750 →	nuclease SbcCD subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,363,207	0	A	T	100.0%	153.2 / NA	51	I761F (ATT→TTT)	SAUSA300_RS06750	nuclease SbcCD subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (49/2);  total (49/2)

GATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAAATTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGAA  >  NC_007793/1363114‑1363303                                                                                              |                                                                                                 gatgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaaa                                                                                            >  1:823453/1‑100 (MQ=255) gatgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaaa                                                                                            >  1:1788572/1‑100 (MQ=255)  ctgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaaat                                                                                           >  1:3178517/2‑100 (MQ=255)    gaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATgg                                                                                         >  1:5412243/1‑100 (MQ=255)    gaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATgg                                                                                         >  1:4597484/1‑100 (MQ=255)            tataGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCt                                                                                 <  1:4226920/100‑1 (MQ=255)               aGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAgg                                                                              >  1:3454642/1‑100 (MQ=255)                gctGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGt                                                                             >  1:2205580/3‑100 (MQ=255)                       tcataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATc                                                                      >  1:1098062/3‑100 (MQ=255)                        gataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:3674471/2‑100 (MQ=255)                        aataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:4046595/1‑100 (MQ=255)                         ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:1450197/1‑100 (MQ=255)                         ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:3678527/1‑100 (MQ=255)                         ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:2046270/1‑100 (MQ=255)                         ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:2678855/1‑100 (MQ=255)                         ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:4304364/1‑100 (MQ=255)                          taGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTGGGTTAGAATCAtt                                                                   >  1:5097736/1‑100 (MQ=255)                           aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAtaa                                                                  >  1:4417316/1‑98 (MQ=255)                           aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                  >  1:326774/1‑100 (MQ=255)                            gCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTaa                                                                 >  1:3070296/1‑100 (MQ=255)                             ccTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAAc                                                                >  1:3020870/1‑100 (MQ=255)                              cTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTAT‑AAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATGAACTa                                                              <  1:4692857/100‑1 (MQ=255)                                 gAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAg                                                            >  1:2284144/1‑100 (MQ=255)                                 cagcggCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAg                                                            >  1:4809285/5‑100 (MQ=255)                                 aaccggCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAg                                                            >  1:2690620/5‑100 (MQ=255)                                            cagagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGtt                                                 >  1:5347626/2‑100 (MQ=255)                                             agagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTa                                                >  1:2979350/1‑100 (MQ=255)                                             agagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTa                                                >  1:3918337/1‑100 (MQ=255)                                             agagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAAGAtcggaagagc                                                >  1:2186343/1‑91 (MQ=255)                                             agagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAAAATCATTAACTAAGGATAAAGAGTTa                                                >  1:4351351/1‑100 (MQ=255)                                                   cagggagCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGAc                                          >  1:4743154/5‑100 (MQ=255)                                                   aTTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTAGAc                                          >  1:2724781/1‑100 (MQ=255)                                                     tAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActc                                        >  1:4426312/1‑100 (MQ=255)                                                     tAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATCAACTAAGGATAAAGAGTTATTGGActc                                        >  1:2248724/1‑100 (MQ=255)                                                     aaggggaGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActc                                        >  1:5155993/7‑100 (MQ=255)                                                      tggggggAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                       >  1:3619186/7‑100 (MQ=255)                                                      ngagCAGAAAAGGAATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                       >  1:1333451/2‑100 (MQ=255)                                                      cgccggggaaaGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                       >  1:362470/9‑100 (MQ=255)                                                      agagCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                       >  1:1587425/1‑100 (MQ=255)                                                       gcccAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTg                                      >  1:647687/4‑100 (MQ=255)                                                        agCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                     >  1:2853568/1‑100 (MQ=255)                                                        aacAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                     >  1:2708345/3‑100 (MQ=255)                                                         gCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:4155558/1‑100 (MQ=255)                                                          cAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATa                                   >  1:1407804/1‑100 (MQ=255)                                                           tgAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaa                                  >  1:2000563/2‑100 (MQ=255)                                                            gAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaaa                                 >  1:389573/1‑100 (MQ=255)                                                            gAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaaa                                 >  1:107717/1‑100 (MQ=255)                                                              caaGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAGtt                               >  1:3027962/2‑100 (MQ=255)                                                               aaGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTa                              >  1:1492577/1‑100 (MQ=255)                                                                                      aTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaat       >  1:4744617/1‑99 (MQ=255)                                                                                      aTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaag       >  1:3268061/1‑99 (MQ=255)                                                                                      aTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaa       >  1:287822/1‑100 (MQ=255)                                                                                       gcATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaata      >  1:2761000/2‑98 (MQ=255)                                                                                       gcATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaata      >  1:2367633/2‑98 (MQ=255)                                                                                           gaatttgaaatGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGaa  <  1:4446854/100‑1 (MQ=255)                                                                                           gaatttgaaatGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGaa  >  1:3560961/1‑100 (MQ=255)                                                                                              |                                                                                                 GATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAAATTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGAA  >  NC_007793/1363114‑1363303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑