Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,295,497	A→G	A207A (GCA→GCG)	SAUSA300_RS06365 →	competence protein CinA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,295,497	0	A	G	100.0%	94.4 / NA	34	A207A (GCA→GCG)	SAUSA300_RS06365	competence protein CinA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/3);  total (31/3)

ACATTCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAC  >  NC_007793/1295401‑1295588                                                                                                 |                                                                                            ctcttCCGATCTATTATGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCt                                                                                          <  1:2388438/97‑1 (MQ=255)          ccTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGa                                                                                 >  1:5324951/2‑100 (MQ=255)               tAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCa                                                                            >  1:3579763/1‑100 (MQ=255)                     ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                      >  1:3777139/1‑100 (MQ=255)                     ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                      >  1:487708/1‑100 (MQ=255)                     ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                      >  1:5404823/1‑100 (MQ=255)                     ctGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                      >  1:422451/2‑100 (MQ=255)                     ctGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                      >  1:2339298/2‑100 (MQ=255)                      tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:990451/1‑100 (MQ=255)                      tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:3546798/1‑100 (MQ=255)                       gCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtat                                                                    >  1:4574028/1‑100 (MQ=255)                       acGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtat                                                                    >  1:940843/2‑100 (MQ=255)                        cGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtata                                                                   >  1:2162575/1‑100 (MQ=255)                            aaTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATAtt                                                               <  1:1111070/100‑1 (MQ=255)                               aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                            >  1:3617624/1‑100 (MQ=255)                                 gTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGAtt                                                          >  1:4422016/1‑100 (MQ=255)                                 gTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGAtt                                                          >  1:4240726/1‑100 (MQ=255)                                         aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGTGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                  >  1:4129017/1‑100 (MQ=255)                                         aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCag                                                  <  1:3600909/100‑2 (MQ=255)                                         aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                  >  1:5154046/1‑100 (MQ=255)                                                                   tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:523462/1‑100 (MQ=255)                                                                   tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:3488138/1‑100 (MQ=255)                                                                   tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:3447103/1‑100 (MQ=255)                                                                   tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:2911126/1‑100 (MQ=255)                                                                   tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:5098551/1‑100 (MQ=255)                                                                   tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:5219804/1‑100 (MQ=255)                                                                                      ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:3793601/1‑100 (MQ=255)                                                                                      ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:667232/1‑100 (MQ=255)                                                                                      ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:4175658/1‑100 (MQ=255)                                                                                      ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:2563538/1‑100 (MQ=255)                                                                                      ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:1905293/1‑100 (MQ=255)                                                                                        gaCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTaaa   >  1:1792883/2‑100 (MQ=255)                                                                                         aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:3420613/1‑100 (MQ=255)                                                                                         aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:2886214/1‑100 (MQ=255)                                                                                                 |                                                                                            ACATTCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAC  >  NC_007793/1295401‑1295588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑