Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,269,542	A→T	I18I (ATA→ATT)	polC →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,269,542	0	A	T	100.0%	130.4 / NA	41	I18I (ATA→ATT)	polC	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (40/1);  total (40/1)

AACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAAT  >  NC_007793/1269450‑1269624                                                                                             |                                                                                   aaCACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttcaaatc                                                                             >  1:4099373/1‑100 (MQ=255)  gcacGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttcaaatca                                                                            >  1:4610513/2‑100 (MQ=255)                                      tCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGac                                        >  1:134111/1‑100 (MQ=255)                                      gcTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGac                                        >  1:4330061/2‑100 (MQ=255)                                       cTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                       >  1:1935874/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:3202158/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:278064/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1612319/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2651837/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2340542/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1962711/1‑100 (MQ=255)                                         tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1931011/1‑100 (MQ=255)                                         tGGCAATGAAAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1911891/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:4207476/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:347236/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:272984/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:1892900/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:4704787/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:4757275/1‑100 (MQ=255)                                          ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:5507560/1‑100 (MQ=255)                                           gCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGta                                   >  1:1830568/1‑100 (MQ=255)                                            ccaTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtat                                  >  1:4422577/3‑100 (MQ=255)                                             gaTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtata                                 >  1:4267684/2‑100 (MQ=255)                                              aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                                >  1:1415638/1‑100 (MQ=255)                                                gACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGAt                              >  1:4973979/1‑100 (MQ=255)                                                gACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGAt                              >  1:30503/1‑100 (MQ=255)                                                gACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGCATAGAt                              >  1:1007470/1‑100 (MQ=255)                                                  ctagtccacaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGt                            >  1:4633231/8‑100 (MQ=255)                                                   ctggCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGtt                           >  1:894533/4‑100 (MQ=255)                                                    tagCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGttt                          >  1:1213765/2‑100 (MQ=255)                                                      gCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCt                        >  1:289308/1‑100 (MQ=255)                                                      gCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCt                        >  1:1746410/1‑100 (MQ=255)                                                         acaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAAc                     >  1:2563489/1‑100 (MQ=255)                                                         acaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAAc                     >  1:1617201/1‑100 (MQ=255)                                                         acaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAAc                     >  1:5365568/1‑100 (MQ=255)                                                               aTTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAAc               <  1:1607130/100‑1 (MQ=255)                                                                       agcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATg       >  1:144798/2‑100 (MQ=255)                                                                        gcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAATTGACACGTATAGATGTTTCTAACAAAAACAGAACATgg      >  1:4544416/1‑100 (MQ=255)                                                                         cttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATggg     >  1:1048380/1‑100 (MQ=255)                                                                           tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGTACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa   >  1:509287/1‑100 (MQ=255)                                                                           tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAAcgtaaaaa   >  1:1792942/1‑93 (MQ=255)                                                                           tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa   >  1:320152/1‑100 (MQ=255)                                                                            gctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGagatcggaaga  >  1:492289/1‑90 (MQ=255)                                                                                             |                                                                                   AACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAAT  >  NC_007793/1269450‑1269624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑