Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,185,887	A→T	K160I (AAA→ATA)	SAUSA300_RS05880 →	S4 RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,185,887	0	A	T	100.0%	41.5 / NA	15	K160I (AAA→ATA)	SAUSA300_RS05880	S4 RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/0);  total (15/0)

CGCGAACAAGTTGGAGATATAATTGTGAATGAACGAATTCAATTTGTTTTGACAAGTAGATTGGAATCATTTATTATGTTAGAATTACAACGTATTAAAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACAGTTAGTTCTTTAAG  >  NC_007793/1185790‑1185983                                                                                                  |                                                                                                 cgcgAACAAGTTGGAGATATAATTGTGAATGAACGAATTCAATTTGTTTTGACAAGTAGATTGGAATCATTTATTATGTTAGAATTACAACGTATTATAg                                                                                                >  1:2018203/1‑100 (MQ=255)                                               ccttGACAAGTAGATTGGAATCATTTATTATGTTAGAATTACAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACa                                                  >  1:1456802/3‑100 (MQ=255)                                                                            atGTTAGAATTACAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTg                     >  1:692040/1‑100 (MQ=255)                                                                               cctgtaTTACAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCaa                  >  1:775941/6‑100 (MQ=255)                                                                                    aTTACAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagtt             >  1:2844968/1‑100 (MQ=255)                                                                                      tACAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttag           >  1:4997908/1‑100 (MQ=255)                                                                                       gcAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttagt          >  1:4524699/2‑100 (MQ=255)                                                                                       aCAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttagt          >  1:1661173/1‑100 (MQ=255)                                                                                       aCAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttagt          >  1:1683085/1‑100 (MQ=255)                                                                                       aCAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttagt          >  1:2077116/1‑100 (MQ=255)                                                                                       aCAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttagt          >  1:3453217/1‑100 (MQ=255)                                                                                       aCAACGTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACagttagt          >  1:4035045/1‑100 (MQ=255)                                                                                          ctgTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACAGTTAGTTCt       >  1:2068641/3‑100 (MQ=255)                                                                                           agTATTATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACAGTTAGTTCtt      >  1:930070/2‑100 (MQ=255)                                                                                               ttATAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACAGTTAGTTCTTTAAg  >  1:4708963/1‑100 (MQ=255)                                                                                                  |                                                                                                 CGCGAACAAGTTGGAGATATAATTGTGAATGAACGAATTCAATTTGTTTTGACAAGTAGATTGGAATCATTTATTATGTTAGAATTACAACGTATTAAAGGCGCATCAGTTAAACTTTATACTATTCCAGTAACAGATATGATACAATCTAATGAGAATTGGAAAAATGAAAGTGCAACAGTTAGTTCTTTAAG  >  NC_007793/1185790‑1185983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑