Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,046,507	C→T	V495I (GTT→ATT)	SAUSA300_RS05135 ←	bifunctional autolysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,046,507	0	C	T	100.0%	223.3 / NA	76	V495I (GTT→ATT)	SAUSA300_RS05135	bifunctional autolysin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/75);  total (1/75)

ATTTAGCTGTGTTGTAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAACAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACAGT  >  NC_007793/1046435‑1046603                                                                         |                                                                                                 aTTTAGCTGTGTTGTAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTTCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGt                                                                       <  1:4798720/100‑1 (MQ=255)        tgtgTTGTAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtt                                                                <  1:3148962/100‑1 (MQ=255)        tgtgTTGTAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtt                                                                <  1:3096949/100‑1 (MQ=255)        tgtgTTGTAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGta                                                                <  1:4795939/100‑2 (MQ=255)          tgttgtAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtttt                                                              <  1:733901/100‑1 (MQ=255)          tgttgtAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtttt                                                              <  1:2626800/100‑1 (MQ=255)          tgttgtAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtttt                                                              <  1:5075283/100‑1 (MQ=255)          tgttgtAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtttt                                                              <  1:3169471/100‑1 (MQ=255)          tgttgtAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGtttt                                                              <  1:2902243/100‑1 (MQ=255)                               tcttAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTg                                         >  1:227596/3‑100 (MQ=255)                                            gaaTTTATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGtc                            <  1:1925404/99‑2 (MQ=255)                                               tttATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTTCCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCt                         <  1:2792901/100‑1 (MQ=255)                                               tttATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCt                         <  1:2286510/100‑1 (MQ=255)                                               tttATTACCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCt                         <  1:1617681/100‑1 (MQ=255)                                                ttattaCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAGTGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCtt                        <  1:5152086/100‑1 (MQ=255)                                                ttattaCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCtt                        <  1:1616019/100‑1 (MQ=255)                                                ttattaCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCtt                        <  1:4925735/100‑1 (MQ=255)                                                ttattaCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCta                        <  1:920667/100‑2 (MQ=255)                                                ttattaCCAGAATTGTAATCTTGAATAAGACAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCtt                        <  1:4562433/100‑1 (MQ=255)                                                 tattaCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCttt                       <  1:301458/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTCCCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:3942005/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:2769536/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:2883501/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:2768070/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:977529/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:5346365/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:4593939/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:4633409/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:209358/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:1737199/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:4948824/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:5072031/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:1393337/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:5184724/100‑1 (MQ=255)                                                    taCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTAcc                    <  1:1263429/100‑1 (MQ=255)                                                    taCCAGAATTGGAATCTTGAAGAATATAGAATTTTTAATTTCCTAATGTAGCTGTTTTAGATTCAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTccc                    <  1:4839156/100‑1 (MQ=255)                                                     aCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCa                   <  1:1028128/100‑1 (MQ=255)                                                     aCCAGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCCTTAccc                   <  1:1152799/100‑2 (MQ=255)                                                        aGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAgag                <  1:2563058/100‑3 (MQ=255)                                                        aGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtt                <  1:2062884/100‑1 (MQ=255)                                                        aGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtt                <  1:2374561/100‑1 (MQ=255)                                                        aGAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtt                <  1:1255050/100‑1 (MQ=255)                                                        aGAATTGTAATCTTAAATAAGATACAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtt                <  1:861180/100‑1 (MQ=255)                                                         gAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGttt               <  1:3465527/100‑1 (MQ=255)                                                         gAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGttt               <  1:948265/100‑1 (MQ=255)                                                         gAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGttt               <  1:1060084/100‑1 (MQ=255)                                                         gAATTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtga               <  1:1927900/100‑3 (MQ=255)                                                         gAATTGGAATCTTGAATAAGATAGAATTTTTGATTTCCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGttt               <  1:1287879/100‑1 (MQ=255)                                                          aaTTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAgggct              <  1:2913589/100‑5 (MQ=255)                                                          aaTTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtttt              <  1:638373/100‑1 (MQ=255)                                                           aTTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGtttgc             <  1:4340088/100‑3 (MQ=255)                                                           aTTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTg             <  1:2085150/100‑1 (MQ=255)                                                           aTTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTg             <  1:3250700/100‑1 (MQ=255)                                                           aTTGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTg             <  1:3248686/100‑1 (MQ=255)                                                            ttGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTgg            <  1:3699295/100‑2 (MQ=255)                                                            ttGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTgc            <  1:819267/100‑2 (MQ=255)                                                            ttGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGt            <  1:36399/100‑1 (MQ=255)                                                            ttGTAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATG‑TTTTTGAACTTCATTAGTTGCTTTACCAGTTTTgag           <  1:1334280/100‑3 (MQ=255)                                                              gTAATCTTGAATAAGATAGAA‑TTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGt         <  1:3141595/100‑1 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCga         <  1:1622529/100‑2 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGt         <  1:100297/100‑1 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGt         <  1:5491530/100‑1 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGt         <  1:1479312/100‑1 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGt         <  1:4496993/100‑1 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGCCGt         <  1:3261145/100‑1 (MQ=255)                                                               tAATCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCAGTAGTTGCTTTACCAGTTTTGTCGt         <  1:2867576/100‑1 (MQ=255)                                                                aaTCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGtc        <  1:2520004/100‑2 (MQ=255)                                                                 aTCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGtat       <  1:4518512/100‑1 (MQ=255)                                                                 aTCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGtat       <  1:3941094/100‑1 (MQ=255)                                                                  tCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGtata      <  1:466600/100‑1 (MQ=255)                                                                  tCTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGtacg      <  1:4179445/100‑3 (MQ=255)                                                                   cTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATAc     <  1:1347497/100‑1 (MQ=255)                                                                   cTTGAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATAc     <  1:3922569/100‑1 (MQ=255)                                                                      gAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACagt  <  1:2076872/100‑1 (MQ=255)                                                                      gAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACagt  <  1:5116828/100‑1 (MQ=255)                                                                      gAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACagt  <  1:721889/100‑1 (MQ=255)                                                                      gAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACagt  <  1:3955255/100‑1 (MQ=255)                                                                      gAATAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACagt  <  1:3467443/100‑1 (MQ=255)                                                                         |                                                                                                 ATTTAGCTGTGTTGTAAACCACATCGCCTTCTTTAACCCAACCAAATTTATTACCAGAATTGTAATCTTGAACAAGATAGAATTTTTGATTACCTAATGTAGCTGTTTTAGATACAGCAAATGTTTTTTGAACTTCATTAGTTGCTTTACCAGTTTTGTCGTATACAGT  >  NC_007793/1046435‑1046603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑