Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	948,776	C→A	A90D (GCT→GAT)	SAUSA300_RS04690 →	ATP‑dependent helicase/deoxyribonuclease subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	948,776	0	C	A	100.0%	24.9 / NA	10	A90D (GCT→GAT)	SAUSA300_RS04690	ATP‑dependent helicase/deoxyribonuclease subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/2);  total (8/2)

GGTAGTTTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGTAGTTATAGCGAACAAAAGTTATCTAAAGCTGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGTGAAAAATT  >  NC_007793/948682‑948875                                                                                               |                                                                                                    ggTAGTTTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGTAGTTATAGCGAACAAAAGTTATCTAAAGATGCaa                                                                                                >  1:3460949/1‑100 (MQ=255)                        gCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGTAGTTATAGCGAACAAAAGTTATCTAAAGATGCAACGGAAATGATGATTTATAACAtt                                                                         >  1:1703641/1‑100 (MQ=255)                        gCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGTAGTTATAGCGAACAAAAGTTATCTAAAGATGCAACGGAAATGATGATTTATAACAtt                                                                         >  1:5224560/1‑100 (MQ=255)                                                               ctgTAGTTATAGCGAACAAAAGTTATCTAAAGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTatcaatca                                  >  1:486659/3‑100 (MQ=255)                                                                                    gTTATCTAAAGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGttt             <  1:5000078/100‑1 (MQ=255)                                                                                    gTTATCTAAAGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGttt             <  1:758798/100‑1 (MQ=255)                                                                                        ccTAAAGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGTg         >  1:5253130/2‑100 (MQ=255)                                                                                           aaaGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCGATCACAAGCAAAATATTATgggctagatcgga      >  1:5235295/1‑90 (MQ=255)                                                                                           aaaGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGTGaaa      >  1:1695763/1‑100 (MQ=255)                                                                                           aaaGATGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGTGaaa      >  1:591130/1‑100 (MQ=255)                                                                                               cTGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGTGAAAAAtt  >  1:4326039/1‑100 (MQ=255)                                                                                               |                                                                                                    GGTAGTTTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGTAGTTATAGCGAACAAAAGTTATCTAAAGCTGCAACGGAAATGATGATTTATAACATTGTTCAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGTGAAAAATT  >  NC_007793/948682‑948875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑