Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	674,737	C→T	P155S (CCT→TCT)	SAUSA300_RS03225 →	haloacid dehalogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	674,737	0	C	T	100.0%	93.1 / NA	30	P155S (CCT→TCT)	SAUSA300_RS03225	haloacid dehalogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/0);  total (30/0)

AAGATTAAACAATTTCGATTACATTCACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATCCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTGGCGATGATGC  >  NC_007793/674644‑674810                                                                                              |                                                                          aaGATTAAACAATTTCGATTACATTCACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTaaaa                                                                     >  1:578314/1‑100 (MQ=255) aaGATTAAACAATTTCGATTACATTCACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTaaaa                                                                     >  1:3843140/1‑100 (MQ=255)                  aTTACATTCACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATAtgaat                                                    >  1:1709910/1‑98 (MQ=255)                     aCATTCACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATAtgattgat                                                 >  1:3917719/1‑100 (MQ=255)                          cACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACt                                            >  1:5486973/1‑100 (MQ=255)                          aaCTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACt                                            >  1:2619679/2‑100 (MQ=255)                           ccTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTa                                           >  1:5471402/2‑100 (MQ=255)                           aCTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTa                                           >  1:2449750/1‑100 (MQ=255)                            cTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAg                                          >  1:4976668/1‑100 (MQ=255)                              tGGTTTGGTGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAggg                                        >  1:3230728/1‑100 (MQ=255)                              tGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAggg                                        >  1:1364582/1‑100 (MQ=255)                              tGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAggg                                        >  1:1044699/1‑100 (MQ=255)                                  ttGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTAt                                    >  1:3499732/1‑100 (MQ=255)                                   tGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTAtt                                   >  1:1369456/1‑100 (MQ=255)                                    gATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTa                                  >  1:22438/1‑100 (MQ=255)                                    caTGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTa                                  >  1:3939828/2‑100 (MQ=255)                                     aTGCATGTTATTAATTATTCATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTAc                                 >  1:3519513/1‑100 (MQ=255)                                      taggtGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTAcc                                >  1:3811548/5‑100 (MQ=255)                                      tGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTAcc                                >  1:2066608/1‑100 (MQ=255)                                      tGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTAcc                                >  1:2797145/1‑100 (MQ=255)                                      ggCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTAcc                                >  1:4862238/2‑100 (MQ=255)                                      agCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTAcc                                >  1:4885908/2‑100 (MQ=255)                                       gCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTATGGGTATTACCt                               >  1:3474739/1‑100 (MQ=255)                                       gCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGtgagatcg                               >  1:4270092/1‑93 (MQ=255)                                       gCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCt                               >  1:2369641/1‑100 (MQ=255)                                                           cAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTgg           >  1:2446101/1‑100 (MQ=255)                                                           cAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTgg           >  1:3690952/1‑100 (MQ=255)                                                           cAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTgg           >  1:3655204/1‑100 (MQ=255)                                                           cAACATCAGAAACAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTgg           >  1:2461837/1‑100 (MQ=255)                                                                    taaCAGTTGGTTTTCGTAAACCACATTCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTGGCGATGATGc  >  1:1296861/2‑100 (MQ=255)                                                                                              |                                                                          AAGATTAAACAATTTCGATTACATTCACTTGGTTTGATGCATGTTATTAATTATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATCCTAAAATTTTTGAAGATATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTGGCGATGATGC  >  NC_007793/674644‑674810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑