Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	556,082	T→C	intergenic (+443/‑115)	SAUSA300_RS02660 → / → SAUSA300_RS02665	lysine‑‑tRNA ligase/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	556,082	0	T	C	100.0%	38.5 / NA	17	intergenic (+443/‑115)	SAUSA300_RS02660/SAUSA300_RS02665	lysine‑‑tRNA ligase/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/0);  total (17/0)

TAGAGGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGTCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACTATTGCACCTTATTAATTAA  >  NC_007793/555990‑556166                                                                                             |                                                                                     tAGAGGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGGATGAAGTAATATATAAAAGTCGCCaaacag                                                                               >  1:4578408/1‑97 (MQ=255)  tgcgcccACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACa                                                                              >  1:4653026/7‑100 (MQ=255)    tgggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCGAAAACAGa                                                                            >  1:3755268/2‑100 (MQ=255)    cgggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAGACAGa                                                                            >  1:2060797/2‑100 (MQ=255)    actgCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCaaaacagat                                                                            >  1:2984689/4‑95 (MQ=255)     gggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAGAACAGat                                                                           >  1:2466884/1‑99 (MQ=255)     ctgCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCGAAAACAGat                                                                           >  1:2638317/3‑99 (MQ=255)     ccgCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCaaccagatcg                                                                           >  1:1421302/3‑92 (MQ=255)      ggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCattcagatcgg                                                                          >  1:2744801/1‑90 (MQ=255)      ggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCaaagatcggaa                                                                          >  1:2252223/1‑92 (MQ=255)      agCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAACGACAGatc                                                                          >  1:5095303/2‑98 (MQ=255)       gCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAACCAGatcg                                                                         >  1:3834369/1‑97 (MQ=255)         ccGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATCTAAAAGTCGCCAAAAACAGatcgga                                                                       >  1:1990339/2‑95 (MQ=255)         ccGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAGCagaagatc                                                                       >  1:110990/2‑98 (MQ=255)           gagaAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAGatcggaag                                                                     >  1:3826780/1‑93 (MQ=255)                                  aaaaaaGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAGACGAAACACACTAAAAGCTGaagatcggaag                                              >  1:547018/1‑90 (MQ=255)                                                                              aaTATATAAAAGTCGCCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACTATTGCACCTTAataattaa  >  1:5355416/1‑100 (MQ=255)                                                                                             |                                                                                     TAGAGGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGTCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACTATTGCACCTTATTAATTAA  >  NC_007793/555990‑556166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑