Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	425,833	A→T	E106D (GAA→GAT)	SAUSA300_RS02000 →	phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	425,833	0	A	T	100.0%	34.4 / NA	13	E106D (GAA→GAT)	SAUSA300_RS02000	phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/1);  total (12/1)

GCATCAACGACTACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGAAAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGTTTTT  >  NC_007793/425741‑425879                                                                                             |                                               gCATCAACGACTACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATAtt                                         >  1:298851/1‑100 (MQ=255) gCATCAACGACTACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATAtt                                         >  1:997694/1‑100 (MQ=255)            tACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCa                              >  1:2570262/1‑100 (MQ=255)             aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                             >  1:1130847/1‑100 (MQ=255)             aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                             >  1:5307099/1‑100 (MQ=255)             aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                             >  1:772238/1‑100 (MQ=255)             aCCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                             >  1:991191/1‑100 (MQ=255)             aCCCCCCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCaa                             >  1:2255407/1‑100 (MQ=255)                                    gcTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGt      >  1:5297188/2‑100 (MQ=255)                                       tGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGtttt   >  1:1445128/1‑100 (MQ=255)                                       tGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGtttt   >  1:22178/1‑100 (MQ=255)                                       tGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGtttt   >  1:2783604/1‑100 (MQ=255)                                        ggCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGATAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGtgcgg  <  1:4896733/100‑5 (MQ=255)                                                                                             |                                               GCATCAACGACTACCCCTCTATTAAGAGAACGTTCACTTGGCGTATTTGAGGGTGAATATAAAGATGAAATCAGTGCGAATCCGAAATATGAAAAATATTTCAATGATCCAAACTTTAAAGACTTTCGTCATAGTTTTT  >  NC_007793/425741‑425879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑