Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,231	T→G	S200R (AGT→CGT)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,231	0	T	G	100.0%	31.0 / NA	13	S200R (AGT→CGT)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/11);  total (2/11)

CTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTG  >  NC_007793/376153‑376314                                                                               |                                                                                    cTAAAATAAGATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCAcc                                                                <  1:2763548/100‑1 (MQ=255)    aaataaaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTa                                                             <  1:1825479/100‑1 (MQ=255)         aaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGt                                                        <  1:2373756/100‑1 (MQ=255)            ttGTCAGGTGCTTCTTTATCAATCTCTTCTTGTCCCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTg                                                     <  1:4005106/100‑1 (MQ=255)            ttGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTg                                                     <  1:1653938/100‑1 (MQ=255)             tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                    <  1:1250974/100‑1 (MQ=255)             tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                    <  1:1984668/100‑1 (MQ=255)             tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                    <  1:4465011/100‑1 (MQ=255)             tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                    <  1:889124/100‑1 (MQ=255)                                 atcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                <  1:2965565/100‑1 (MQ=255)                                             aCCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGttt                    >  1:1462621/1‑100 (MQ=255)                                                  ccgcaTAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATc               >  1:3864175/2‑100 (MQ=255)                                                               aaaCATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTtgtg  <  1:3301568/100‑1 (MQ=255)                                                                               |                                                                                    CTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTG  >  NC_007793/376153‑376314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑