Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,057	C→T	D258N (GAC→AAC)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,057	0	C	T	100.0%	13.9 / NA	7	D258N (GAC→AAC)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/6);  total (1/6)

TTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGC  >  NC_007793/376008‑376144                                                  |                                                                                        ttttGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGtat                                       <  1:1524965/100‑1 (MQ=255)             tGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATCGGTTGAACTCGTCAATGGTATATCCTAAATCag                           <  1:1276714/100‑2 (MQ=255)                   actcAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctc                     >  1:4411659/4‑100 (MQ=255)                                  gCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCg      <  1:5551662/100‑1 (MQ=255)                                   cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt     <  1:3474624/100‑1 (MQ=255)                                    tAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtt    <  1:4142653/100‑1 (MQ=255)                                    tAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtt    <  1:797563/100‑1 (MQ=255)                                      aGTATTGGATGTTTGAAACGTCCATGACCCAACTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGCTGTTga  <  1:5412276/100‑2 (MQ=255)                                                  |                                                                                        TTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGC  >  NC_007793/376008‑376144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑