Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	292,738	A→C	T341P (ACG→CCG)	SAUSA300_RS01295 →	sorbitol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	292,738	0	A	C	100.0%	22.4 / NA	10	T341P (ACG→CCG)	SAUSA300_RS01295	sorbitol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/4);  total (6/4)

TGGTGACAAAAATATATGATATTTCGAAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGACGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAGAATCAATGCTAACTTTTATGCTTGGGCCATTAAGACAAATCACTGA  >  NC_007793/292652‑292827                                                                                       |                                                                                          tGGTGACAAAAATATATGATATTTCGAAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGa                                                                              >  1:2183116/1‑100 (MQ=255) tGGTGACAAAAATATATGATATTTCGAAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGa                                                                              >  1:4050559/1‑100 (MQ=255)     gACAAAAATATATGATATTTCGAAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGaa                                                                          >  1:2305226/1‑100 (MQ=255)               tatGATATTTCGAAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAATGAAGgag                                                                <  1:1297334/100‑1 (MQ=255)                               gggACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAg                                                >  1:1558966/1‑100 (MQ=255)                                 gACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAggc                                              <  1:1865207/100‑3 (MQ=255)                                  ccGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAGAAt                                             >  1:5523044/2‑100 (MQ=255)                                         gTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAGAATCAATGCt                                      <  1:3250994/100‑1 (MQ=255)                                                               gAAGGTATTAAAGTATTACTTAAGCCGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAGAATCAATGCTAACTTTTATGCTTGGGCCATTa                <  1:3436603/100‑1 (MQ=255)                                                                             aTTACTTAAGCCGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAGAATCAATGCTAACTTTTATGCTTGGGCCATTAAGACAAATCACTGa  >  1:4105886/1‑100 (MQ=255)                                                                                       |                                                                                          TGGTGACAAAAATATATGATATTTCGAAATGGGACGAGGCGTATCAACATTTAAAATCCGGCGAAGGTATTAAAGTATTACTTAAGACGCTCGATTTAGATGAAAATGAAGGAGAGAATTAATATGGTAGAATCAATGCTAACTTTTATGCTTGGGCCATTAAGACAAATCACTGA  >  NC_007793/292652‑292827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑