Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,249,0090AT73.3% 12.5 / 5.0 15intergenic (‑396/+3)SAUSA300_RS11480/SAUSA300_RS11485hypothetical protein/ATP‑grasp domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base T (0/11);  total (3/12)
Fisher's exact test for biased strand distribution p-value = 8.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

ACTAGAATCGAAAAAAGCTTGTTACAAACGCATATTCAGTCAACTACTGGCAACACAACATTGTAGCGCCTTATGTTCCGAAC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTGAT  >  NC_007793/2248928‑2249102
                                                                                 |                                                                                              
aCTAGAATCGAAAAAAGCTTGTTACAAACGCATATTCAGTCAACTACTGGCAACACAACATTGTAGCGCCTTATGTTCCGAAC‑TCTAATAGTGTTCAAGt                                                                             >  1:5237074/1‑100 (MQ=255)
                                              ctGGCAACACAACATTGTAGCGCCTTATGTTCCGAAC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAAt                               >  1:4002379/1‑100 (MQ=255)
                                                  caacacaacaTTGTAGCGCCTTATGTTCCGAAC‑TCTAATAGGGTTCAAGTTCAGCTAAAAATGCGCGTTCAGTCTCCAGTACTGTTTCTTTTGAATCAcc                           >  1:1512112/1‑100 (MQ=255)
                                                                  cgtgtgctcttCCGATC‑TCTATTAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCa           <  1:3457926/91‑1 (MQ=255)
                                                                  cgtgtgctcttCCGATC‑TCTAGTAGTGTTCAAGTTCAGCTAAGAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACGGCCa           <  1:41244/91‑1 (MQ=255)
                                                                  cgtgtgctcttCCGATC‑TCTAATGGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATAACCATGCCAAATACAGCCa           <  1:1844919/91‑1 (MQ=255)
                                                                  cgtgtgctcttCCGATC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCa           <  1:1796911/91‑1 (MQ=255)
                                                                  cgtgtgctcttCCGATC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCa           <  1:2723460/91‑1 (MQ=255)
                                                                  cgtgtgctcttCCGATC‑TCCTGTAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCa           <  1:3118901/91‑1 (MQ=255)
                                                                  cgcCTTATGTTCCGAAC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGccc           <  1:1240923/100‑2 (MQ=255)
                                                                   gtgtgctcttCCGATC‑TCTTATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCaa          <  1:4301245/92‑1 (MQ=255)
                                                                   gtgtgctcttCCGATC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCaa          <  1:5176581/92‑1 (MQ=255)
                                                                         tcttCCGATC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTg    <  1:1372302/98‑1 (MQ=255)
                                                                           ttCCGATCTTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTgg   <  1:759575/100‑2 (MQ=255)
                                                                           ttCCGATC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTGAt  <  1:3418390/100‑1 (MQ=255)
                                                                                 |                                                                                              
ACTAGAATCGAAAAAAGCTTGTTACAAACGCATATTCAGTCAACTACTGGCAACACAACATTGTAGCGCCTTATGTTCCGAAC‑TCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTGAT  >  NC_007793/2248928‑2249102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑