Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,111,0400AT65.7% 575.8 / inf 513E702D (GAA→GAT) SAUSA300_RS05455pyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base A (166/7);  new base T (332/0);  total (506/7)
Fisher's exact test for biased strand distribution p-value = 5.11e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01

Only 100 of 528 total aligned reads displayed.

CTCAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGAT  >  NC_007793/1110941‑1111139
                                                                                                   |                                                                                                   
ctcAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGaa                                                                                                     >  1:3986236/1‑100 (MQ=255)
ctcAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGaa                                                                                                     >  1:3073468/1‑100 (MQ=255)
ctcAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGaa                                                                                                     >  1:268309/1‑100 (MQ=255)
  taGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:1737129/2‑100 (MQ=255)
  taGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:3814253/2‑100 (MQ=255)
  taGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:2585785/2‑100 (MQ=255)
  naGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:2533247/2‑100 (MQ=255)
  cggAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:533478/3‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGGTCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:4455354/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACTTTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTGAGATCg                                                                                                   >  1:4296745/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATCCTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:569942/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:111805/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAATGAGTTAGATCg                                                                                                   >  1:2146136/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGGGTTAGATCg                                                                                                   >  1:1897429/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:1448939/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:2935065/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:1208222/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:616552/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:2373153/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:2305463/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:2023185/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:208823/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCg                                                                                                   >  1:4756329/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTCAGATCg                                                                                                   >  1:4875284/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGCTAGATCg                                                                                                   >  1:3426843/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGATAGATCg                                                                                                   >  1:177779/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAACGAGTTAGATCg                                                                                                   >  1:3659639/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAATAGTTAGATCg                                                                                                   >  1:3648427/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAATAGTTAGATCg                                                                                                   >  1:2647514/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGGGTTAGATCg                                                                                                   >  1:104583/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGGGTTAGATCg                                                                                                   >  1:4389862/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGCGTGAGATCg                                                                                                   >  1:2987179/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGGACg                                                                                                   >  1:1869444/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:947681/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:4709993/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:3525401/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:1003099/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:724870/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:653899/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:41053/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5487602/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5354348/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5128965/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:4429952/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5077317/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:5030477/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:4548066/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:2191115/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:2261874/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:16692/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:285708/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCg                                                                                                   >  1:2887865/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAGCg                                                                                                   >  1:4982853/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1545711/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:179139/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1919708/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:4820781/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:4575445/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1686318/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:2432102/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:693983/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:3468770/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:3346185/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:756811/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:3696029/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1267266/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:1503935/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:148145/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACg                                                                                                   >  1:3904969/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTGAGATCg                                                                                                   >  1:3252398/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTCAGATCg                                                                                                   >  1:1622377/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTCAGATCg                                                                                                   >  1:889743/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTAAGATCg                                                                                                   >  1:2332086/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTAAGATCg                                                                                                   >  1:3386955/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGGTAGATCg                                                                                                   >  1:2467885/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGCTAGATCg                                                                                                   >  1:3166648/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGATAGATCg                                                                                                   >  1:3129589/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGACTTAGATCg                                                                                                   >  1:542403/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGACTCAGATCg                                                                                                   >  1:4182700/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATTAGATCg                                                                                                   >  1:4658099/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATTAGATCg                                                                                                   >  1:37472/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAATTAGATCg                                                                                                   >  1:378407/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAG‑GTTAGATCgg                                                                                                  >  1:4944928/1‑99 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAGTCAGATCg                                                                                                   >  1:824336/1‑100 (MQ=255)
  cAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAAAATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAGCg                                                                                                   >  1:4063742/1‑100 (MQ=255)
  cAGAAGGTACTAGTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGACTTAGAACg                                                                                                   >  1:4259628/1‑100 (MQ=255)
  cAGAAGGGACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAAAATTAGATCg                                                                                                   >  1:1157733/1‑100 (MQ=255)
     gaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGAATTATGTCAAACTAGCTAATGAGTTAGATCggaa                                                                                                >  1:3222047/2‑97 (MQ=255)
     caGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGATCggaa                                                                                                >  1:5170013/2‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAACGAGTTAGATCggaa                                                                                                >  1:3551586/1‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAATGGTTAGATCggaa                                                                                                >  1:4367865/1‑97 (MQ=255)
     aaGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGa                                                                                                >  1:528890/1‑100 (MQ=255)
       ggTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCggaaga                                                                                              >  1:3886410/1‑95 (MQ=255)
       ggTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTCAGATCggaaga                                                                                              >  1:1824589/1‑95 (MQ=255)
        gTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAGGAGTTAGATCggaagag                                                                                             >  1:5226398/1‑94 (MQ=255)
                     taCAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTa                                                                                >  1:1302504/1‑100 (MQ=255)
                                                         ttATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGCTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTaat                                            <  1:5532661/100‑2 (MQ=255)
                                                                                 gcTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAg                    >  1:4511013/2‑100 (MQ=255)
                                                                                  gcaGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGt                   >  1:4322277/3‑100 (MQ=255)
                                                                                              agcacaCGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAAACTAAAGCCGCTTACGAATTAATTGGTGAGTTAAAATCAGCTg       >  1:2716503/6‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTCAGAAGGTACTATTTGTTATACAGGTGACATTTTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGAT  >  NC_007793/1110941‑1111139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑