Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,150,261	+C	coding (766/960 nt)	SAUSA300_RS10960 ←	fructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,150,257	1	.	C	96.0%	207.3 / 3.3	74	coding (770/960 nt)	SAUSA300_RS10960	fructokinase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (3/68);  total (3/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCTAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑CCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCACCATCTGCTCCCTTCGTAT  >  NC_007793/2150158‑2150353                                                                                                     |                                                                                                  gCTAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTg                                                                                                    <  1:3018143/100‑1 (MQ=255)  ctcttccgatcTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑c                                                                                                  <  1:1639192/90‑1 (MQ=255)  cTAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTgg                                                                                                   <  1:5434511/100‑2 (MQ=255)   tAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑cg                                                                                                 <  1:2203077/100‑2 (MQ=255)   tAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑cc                                                                                                 <  1:140026/100‑1 (MQ=255)   tAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ca                                                                                                 <  1:3230564/100‑2 (MQ=255)   tAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ca                                                                                                 <  1:2186649/100‑2 (MQ=255)        tttCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccag                                                                                           <  1:2392129/100‑4 (MQ=255)        tttCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccag                                                                                           <  1:2166916/100‑4 (MQ=255)         ttCCTCGCCTTCATTTTCAAATAACTCAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCATTAAATGCATCCCCTG‑ccc‑ccagt                                                                                          <  1:4350985/100‑5 (MQ=255)         ttCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagt                                                                                          <  1:4572004/100‑5 (MQ=255)         ttCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagt                                                                                          <  1:3034826/100‑5 (MQ=255)         ttCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagt                                                                                          <  1:2091097/100‑5 (MQ=255)         ttCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagg                                                                                          <  1:3783179/100‑5 (MQ=255)         tctcTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagt                                                                                          <  1:5506521/97‑5 (MQ=255)          tCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCTGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagtt                                                                                         <  1:1219221/100‑6 (MQ=255)          gatctaCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagtg                                                                                         <  1:3985181/95‑6 (MQ=255)          ctcTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑ccc‑ccagta                                                                                         <  1:122336/98‑6 (MQ=255)           ccTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTg                                                                                         <  1:545796/100‑1 (MQ=255)           ccTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTg                                                                                         <  1:2196192/100‑1 (MQ=255)            ttCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:3284022/99‑1 (MQ=255)            ttCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:3213581/99‑1 (MQ=255)            tctaCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:5406234/97‑1 (MQ=255)            gatctcctCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:1650537/93‑1 (MQ=255)            cgatctttCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:3897448/94‑1 (MQ=255)            ccgatcTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATTACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:922282/95‑1 (MQ=255)            cTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTga                                                                                        <  1:1745189/100‑2 (MQ=255)            cTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:3561677/100‑1 (MQ=255)            cTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:807526/100‑1 (MQ=255)            cTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:846410/100‑1 (MQ=255)            cTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:973296/100‑1 (MQ=255)            atctccTTCGTTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:1006212/96‑1 (MQ=255)            atctccTTCATTTTCATATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:1699936/96‑1 (MQ=255)            atctccTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGt                                                                                        <  1:1931783/96‑1 (MQ=255)              tctcTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAGTCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTAt                                                                                      <  1:2458564/97‑1 (MQ=255)              cACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTag                                                                                      <  1:3826372/100‑2 (MQ=255)              cACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTaa                                                                                      <  1:1995210/100‑2 (MQ=255)               tccTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:1193784/99‑1 (MQ=255)               ctcTTCATTATCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:3353042/98‑1 (MQ=255)               aCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTAta                                                                                     <  1:4712415/100‑2 (MQ=255)               aCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:1072875/100‑1 (MQ=255)               aCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:3526047/100‑1 (MQ=255)               aCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:746615/100‑1 (MQ=255)               aCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:3254800/100‑1 (MQ=255)               aCCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATc                                                                                     <  1:4941702/100‑1 (MQ=255)                ccTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAGTAAATGCATCCCCTGCCCC‑CAGTTGTATCa                                                                                    <  1:250641/100‑1 (MQ=255)                ccTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCa                                                                                    <  1:2081437/100‑1 (MQ=255)                ccTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCa                                                                                    <  1:2529013/100‑1 (MQ=255)                ccTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCa                                                                                    <  1:2987927/100‑1 (MQ=255)                 cTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCac                                                                                   <  1:1181944/100‑2 (MQ=255)                 cTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCaa                                                                                   <  1:1944197/100‑1 (MQ=255)                      ttttCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAgga                                                                              <  1:1646395/100‑2 (MQ=255)                                aCTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCGTCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTtat                                                                    <  1:1414882/100‑1 (MQ=255)                                aCTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTtat                                                                    <  1:5482870/100‑1 (MQ=255)                                 cTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTCACCTtatg                                                                   <  1:455165/100‑2 (MQ=255)                                    aGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGcc                                                                <  1:3594105/100‑1 (MQ=255)                                    aGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGcc                                                                <  1:1750164/100‑1 (MQ=255)                                    aGTTAAATTTAACACATCGGTCGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTGCCTTATAGcc                                                                <  1:2847468/100‑1 (MQ=255)                                      ttAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCAc                                                              <  1:4757332/100‑1 (MQ=255)                                       tAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACt                                                             <  1:2981665/100‑1 (MQ=255)                                       tAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACt                                                             <  1:3286619/100‑1 (MQ=255)                                       tAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACt                                                             <  1:4248510/100‑1 (MQ=255)                                       tAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTCTTACCTTATAGccgca                                                             <  1:2004135/100‑4 (MQ=255)                                        aaaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCagat                                                            <  1:3437886/100‑4 (MQ=255)                                        aaaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCAcga                                                            <  1:3962756/100‑3 (MQ=255)                                        aaaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACtg                                                            <  1:3832339/100‑2 (MQ=255)                                        aaaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTa                                                            <  1:3141153/100‑1 (MQ=255)                                        aaaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTa                                                            <  1:5216329/100‑1 (MQ=255)                                        aaaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑CCCTCAGTTGTATCAACAGGTTTTACCTTATAGCCACTa                                                            <  1:2828179/100‑1 (MQ=255)                                         aaTTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTAt                                                           <  1:4964075/100‑1 (MQ=255)                                                   aTCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGAtt                                                 <  1:2147141/100‑1 (MQ=255)                                                       gTTGCTAAAATCCTGCTAATAACTGCACCAATAGATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATa                                             >  1:2933826/1‑100 (MQ=255)                                                         tGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATAcc                                           <  1:736631/100‑1 (MQ=255)                                                         tGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATAcc                                           <  1:334426/100‑1 (MQ=255)                                                                  ccTGCTAATAACTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCaa                                  >  1:2037950/1‑100 (MQ=255)                                                                           atcTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:2677764/98‑1 (MQ=255)                                                                           aaCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:5040634/100‑1 (MQ=255)                                                                           aaCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:169308/100‑1 (MQ=255)                                                                           aaCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:5318183/100‑1 (MQ=255)                                                                           aaCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:87780/100‑1 (MQ=255)                                                                           aaCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:3200168/100‑1 (MQ=255)                                                                           aaCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgc                         <  1:1396661/100‑1 (MQ=255)                                                                            aCTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACggag                        <  1:3341603/100‑3 (MQ=255)                                                                             cTGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAAAGATTAATACCATTTTTCAAATATACGgcaa                       <  1:261284/100‑2 (MQ=255)                                                                              tGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgcagc                      <  1:5381832/100‑1 (MQ=255)                                                                              tGCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgcagc                      <  1:3468489/100‑1 (MQ=255)                                                                               gCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTTCCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgcagat                     <  1:647004/100‑3 (MQ=255)                                                                               gCACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgcagca                     >  1:3655011/1‑100 (MQ=255)                                                                                cACCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGgcagcaa                    <  1:1766250/100‑2 (MQ=255)                                                                                 aCCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCAcc                   <  1:4695368/100‑1 (MQ=255)                                                                                 aCCAATAAATGCATCCCCTGCCCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCAcc                   <  1:3500209/100‑1 (MQ=255)                                                                                            tccgatcTG‑CCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCACCATCTGCTCCCtt       <  1:1431623/94‑1 (MQ=255)                                                                                                 tcTG‑CCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCACCATCTGCTCCCTTCGtag  <  1:3132012/99‑2 (MQ=255)                                                                                                 tcTG‑CCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCACCATCTGCTCCCTTCGtag  <  1:2677955/99‑2 (MQ=255)                                                                                                     |                                                                                                  GCTAAAATTTCCTCACCTTCATTTTCAAATAACTGAGTTAAATTTAACACATCGGTTGCTAAAATCCTGCTAATAACTGCACCAATAAATGCATCCCCTG‑CCC‑CAGTTGTATCAACAGGTTTTACCTTATAGCCACTATGATAATGATTAATACCATTTTTCAAATATACGGCAGCACCATCTGCTCCCTTCGTAT  >  NC_007793/2150158‑2150353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑