| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 2,000,301 | 4 bp→ACGA | noncoding (394‑397/2926 nt) | SAUSA300_RS10040 ← | 23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 2,000,301 | 0 | C | A | 95.2% | 20.7 / ‑4.1 | 21 | noncoding (397/2926 nt) | SAUSA300_RS10040 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (19/1); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
| * | NC_007793 | 2,000,302 | 0 | A | C | 95.2% | 20.1 / ‑3.9 | 21 | noncoding (396/2926 nt) | SAUSA300_RS10040 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (19/1); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
| * | NC_007793 | 2,000,303 | 0 | A | G | 95.2% | 19.6 / ‑4.4 | 21 | noncoding (395/2926 nt) | SAUSA300_RS10040 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (19/1); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-01 | |||||||||||
| * | NC_007793 | 2,000,304 | 0 | C | A | 95.2% | 20.1 / ‑3.7 | 21 | noncoding (394/2926 nt) | SAUSA300_RS10040 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (19/1); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-01 | |||||||||||
ATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATGTC > NC_007793/2000220‑2000366 |||| aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgg > 1:2042896/1‑100 (MQ=11)aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAga > 1:1718859/1‑99 (MQ=11)aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACATGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgg > 1:2371059/1‑100 (MQ=11) ttcaGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAggg > 1:2840595/4‑99 (MQ=11) ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCATGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc > 1:2641407/1‑98 (MQ=11) ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTTAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc > 1:6303581/1‑98 (MQ=11) ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACCCAGGAGAGAACGAAGTTTTGACTACAgggc > 1:531210/1‑98 (MQ=11) tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct > 1:2566322/1‑97 (MQ=11) tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct > 1:1070083/1‑97 (MQ=11) tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct > 1:2359160/1‑97 (MQ=11) tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggca > 1:4383481/1‑97 (MQ=11) tAGCCTTGGAAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct > 1:33654/1‑97 (MQ=11) aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGGGAACGAAGTTTTGACTACAgggctg > 1:2268379/1‑96 (MQ=11) aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg > 1:2429999/1‑96 (MQ=11) aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg > 1:3726397/1‑96 (MQ=11) aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGCGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAggggca > 1:2423555/1‑96 (MQ=11) aTGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTAcctcctat > 1:5285011/1‑98 (MQ=2) cctccCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTCCTATggcgg > 1:2924857/1‑96 (MQ=2) cctccCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTCCTATggcgg > 1:1439884/1‑96 (MQ=2) cccGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTCCTATggcgggcc < 1:1450552/100‑8 (MQ=2) tgtTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATgtc < 1:305466/100‑1 (MQ=32) |||| ATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATGTC > NC_007793/2000220‑2000366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |