Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,332	2 bp→AG	coding (95‑96/357 nt)	SAUSA300_RS08070 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,332	0	T	A	100.0%	26.3 / NA	11	L32F (TTA→TTT)	SAUSA300_RS08070	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/9);  total (2/9)
*	NC_007793	1,632,333	0	A	G	90.9%	19.4 / ‑5.4	11	L32S (TTA→TCA)	SAUSA300_RS08070	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (2/8);  total (2/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCA  >  NC_007793/1632309‑1632426                        ||                                                                                              gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa                    <  1:1086139/91‑1 (MQ=255) gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa                    >  1:1602665/10‑100 (MQ=255)     cactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATAc                >  1:5142556/6‑100 (MQ=255)        tcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaaa             <  1:5608163/98‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc           <  1:1909989/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc           <  1:5343077/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc           <  1:6059737/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa          <  1:1700747/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa          <  1:4145220/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCGTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa          <  1:2392353/100‑1 (MQ=255)                   ctttCAAAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCa  <  1:1774560/99‑1 (MQ=255)                        ||                                                                                              ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCA  >  NC_007793/1632309‑1632426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑