Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,580,758	2 bp→CA	coding (41‑42/258 nt)	SAUSA300_RS07750 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,580,758	0	T	C	100.0%	47.7 / NA	19	T14T (ACA→ACG)	SAUSA300_RS07750	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/17);  total (2/17)
*	NC_007793	1,580,759	0	G	A	84.2%	33.2 / ‑0.6	19	T14I (ACA→ATA)	SAUSA300_RS07750	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (2/14);  total (2/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCAGGTTGTTTAACATTGTCTTGTGTTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTGGTTATAAGTGACATCGAACCAGT  >  NC_007793/1580668‑1580851                                                                                           ||                                                                                             tggtggGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATttcattc                                                                                      <  1:2660787/100‑1 (MQ=255)            gtgCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACttt                                                                           <  1:3126374/100‑1 (MQ=255)               ccGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCt                                                                        <  1:2078570/100‑1 (MQ=255)                  tAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGtt                                                                     <  1:2834991/100‑1 (MQ=255)                    aCTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTAc                                                                   <  1:6065232/100‑1 (MQ=255)                      ttGTTCTATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTg                                                                 <  1:2541841/100‑1 (MQ=255)                      ttGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTg                                                                 <  1:6548683/100‑1 (MQ=255)                      ttGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTg                                                                 <  1:5751879/100‑1 (MQ=255)                         ttCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGAtt                                                              <  1:1804458/100‑1 (MQ=255)                         ttCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTGAtt                                                              <  1:709153/100‑1 (MQ=255)                                  aTCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCAtt                                                     <  1:1173737/100‑1 (MQ=255)                                             cAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACta                                          <  1:5932074/100‑2 (MQ=255)                                               aTGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCa                                        <  1:5584484/100‑1 (MQ=255)                                                    cccGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCt                                   >  1:1832806/2‑100 (MQ=255)                                                            tgtAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACAtt                           <  1:4114413/100‑1 (MQ=255)                                                              tAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTg                         <  1:5425144/100‑1 (MQ=255)                                                               aaTGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACagcgag                        <  1:2799928/100‑6 (MQ=255)                                                                   tgctggTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTGGTtat                    >  1:5759450/5‑100 (MQ=255)                                                                                     atCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTGGTTATAAGTGACATCGAACCAGt  <  1:2076139/99‑1 (MQ=255)                                                                                           ||                                                                                             TGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCAGGTTGTTTAACATTGTCTTGTGTTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTGGTTATAAGTGACATCGAACCAGT  >  NC_007793/1580668‑1580851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑