| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 616,786 | 6 bp→CAATGA | coding (2430‑2435/4146 nt) | SAUSA300_RS02920 → | serine‑aspartate repeat‑containing protein D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 616,786 | 0 | T | C | 86.4% | 28.1 / 4.8 | 44 | D810D (GAT→GAC) | SAUSA300_RS02920 | serine‑aspartate repeat‑containing protein D |
| Reads supporting (aligned to +/- strand): ref base T (5/1); new base C (38/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
| * | NC_007793 | 616,787 | 0 | T | A | 86.4% | 25.6 / 7.6 | 44 | S811T (TCA→ACA) | SAUSA300_RS02920 | serine‑aspartate repeat‑containing protein D |
| Reads supporting (aligned to +/- strand): ref base T (5/1); new base A (38/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
| * | NC_007793 | 616,788 | 0 | C | A | 86.4% | 26.6 / 7.3 | 44 | S811* (TCA→TAA) | SAUSA300_RS02920 | serine‑aspartate repeat‑containing protein D |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (38/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_007793 | 616,789 | 0 | A | T | 86.4% | 28.1 / 5.5 | 44 | S811S (TCA→TCT) | SAUSA300_RS02920 | serine‑aspartate repeat‑containing protein D |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base T (38/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_007793 | 616,790 | 0 | A | G | 86.4% | 28.4 / 4.9 | 44 | T812A (ACT→GCT) | SAUSA300_RS02920 | serine‑aspartate repeat‑containing protein D |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base G (38/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_007793 | 616,791 | 0 | C | A | 86.4% | 26.6 / 7.3 | 44 | T812N (ACT→AAT) | SAUSA300_RS02920 | serine‑aspartate repeat‑containing protein D |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (38/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
GACATTAGATAGTGGATTCTACAAAACAC‑‑‑CAAAATATAATTTAGGTAATTATGTATGGGAAGATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGTTACATTGAAAAATGAAAACGGTGAAGTTTTACAAACAACTAAAACAGATAAAGATGGTAA > NC_007793/616699‑616878 |||||| gACATTAGATAGTGGATTCTACAAAACAC‑‑‑CAAAATATAATTTAGGTAATTATGTATGGGAAGATACAAATAAAGATGGTAAGCAGAATTCAACTGaaaaa > 1:4827596/1‑100 (MQ=255) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:4724715/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:1379578/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:2174082/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:458013/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:6087349/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:598353/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:5867380/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:3017529/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:5451988/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:5568816/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:5595585/1‑100 (MQ=9) tAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGAGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:5428455/1‑100 (MQ=9) gtgTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:5520636/3‑100 (MQ=9) gaGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtt > 1:6236295/2‑100 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAGATACAGCTTAGGTGATTATGTATGGGACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:1982653/1‑99 (MQ=2) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTATGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:3317493/1‑99 (MQ=2) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:5669408/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:5767901/1‑99 (MQ=2) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:6302212/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:5089173/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:6352395/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:6413507/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:790008/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:3146026/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:2160550/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:2165247/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:230942/1‑99 (MQ=2) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:2394953/1‑99 (MQ=2) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:2576655/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:280835/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:2811448/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:3098494/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:4761131/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:3385712/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:3691568/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:3889604/1‑99 (MQ=9) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:4218874/1‑99 (MQ=2) aGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTAtta > 1:4616051/1‑99 (MQ=9) aCAAAACAC‑‑‑CAAAATATAATTTAGGTAATTATGTATGGGAAGATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGt > 1:3409248/1‑100 (MQ=255) tatgGGAAGATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGTTACATTGAAAAATGAAAACGGTGAAGTTTTaca > 1:4858338/1‑100 (MQ=255) gATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGTTACATTGAAAAATGAAAACGGTGAAGTTTTACAAACAACTa < 1:3410092/100‑1 (MQ=255) gATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGTTACATTGAAAAATGAAAACGGTGAAGTTTTACAAACAACTa > 1:5147447/1‑100 (MQ=255) aGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGTTACATTGAAAAATGAAAACGGTGAAGTTTTACAAACAACTAAAACAGATAAAGAtcggaa > 1:2181843/1‑95 (MQ=255) |||||| GACATTAGATAGTGGATTCTACAAAACAC‑‑‑CAAAATATAATTTAGGTAATTATGTATGGGAAGATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACAGTTACATTGAAAAATGAAAACGGTGAAGTTTTACAAACAACTAAAACAGATAAAGATGGTAA > NC_007793/616699‑616878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |